FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5440165
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT85670.15747684123551398No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC74350.13666864883693786No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG62200.11433476741973818No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA59900.11010695447656459No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA55570.10214763706615516No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC55050.10119178370508981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG807.892595E-963.64096145
TCGTTCG1300.061.5429145
GGTCGCG1500.058.186024145
TATCGCG855.2446085E-542.78384145
AGCGAAA11400.040.07671
CGAAAGC15250.039.9302372
GCATTAA42650.035.707261
GCGAAAG17600.035.023741
CCCTTAT29500.033.924491
AGGTTCG1102.3921703E-433.060238145
CATTAAG47000.032.544462
CGTTGCG950.00465562730.624222145
TCGCCGG2151.7578714E-830.446175145
TCGGTCG1204.0709935E-430.1948914
GTAGAAA42100.028.0777131
GTCTAGT27300.026.2983681
TTATACT49850.025.8761464
TAGTACC28550.025.128924
GCTATTG36150.025.0603058
GTATACC18650.024.8682884
Produced by FastQC (version 0.11.5)