FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5836721
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT114350.1959147953105862No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC113740.19486968796349868No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC66750.11436215642310127No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC65500.11222054300693832No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC64580.1106443155326424No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT64270.11011319540543399No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA60970.1044593359867638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16450.061.2675131
GCGAAAG19100.053.131182
TGACGCG2050.049.66873145
CGAAAGC20800.049.1279563
CCGACGG1901.2551027E-1038.27854145
TGTATCG2750.037.025784145
TATACTG38850.036.1883475
TTAGGTA26600.032.4196624
TATGTCG1352.0981845E-532.3241145
CTAGTAC16900.030.8758343
TAGTACT23000.029.9322194
ATACACG3850.026.44699145
TGCGTTA2559.533687E-825.5796078
GACGACG3159.986252E-1025.397507145
GCTATAT13650.024.9658621
GCGGAAT3201.2478267E-924.91712
GGTCGCG2751.9128129E-723.80229145
TAGGTAG41800.023.7521175
CAATTCG3102.431625E-823.379219
GTAGAAA30250.023.2502561
Produced by FastQC (version 0.11.5)