FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R2_A03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R2_A03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379940
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG789691.8029699036973108No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA581171.3268903226984845No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA509401.1630296305428842No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA456731.0427768416918952No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC432340.9870911473673156No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG412310.9413599273049402No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT386300.8819755521765138No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT383160.8748065041986877No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT370290.845422540034795No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG278920.6368123764252478No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC278240.6352598437421517No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC269110.6144148093352877No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT268040.61197185349571No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG254600.581286501641575No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC224710.5130435576743061No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC221010.5045959533692242No Hit
CAAACAGCAGACAAGAGAATAATGGACATGATTCCAGAGACACATCAGGA193250.441216089718124No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA191900.4381338557149185No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT187940.42909263597218233No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC187270.42756293465207285No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG173130.3952793873888683No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC157210.3589318575140299No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA152280.347675995561583No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG144290.3294337365352037No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT142100.32443366804111473No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG140280.32027835997753396No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC137260.3133832883555483No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC135900.310278222989356No Hit
CAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGA133170.30404526089398487No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA131930.3012141718836331No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT130740.2984972396882149No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC129510.29568898204084987No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA128690.2938168102759398No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA127670.2914880112512957No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG127120.29023228628702674No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC122060.27867961661575275No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG120810.27582569624241426No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC120040.2740676812924378No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG117310.2678347191970666No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG116420.26580272789124965No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT114310.2609853103010543No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA114160.2606428398562537No Hit
GAATGAAGTGGATGATGGCAATGGCCCAGCATTAAGCATCAATGAACTGA114070.26043735758937336No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG113590.2593414521660114No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA106810.24386178806102368No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG106000.24201244765910038No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA102340.2336561688059654No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA95240.21744590108540301No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG94300.21529975296465248No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT92380.21091613127120465No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA91640.20922661041018828No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA90930.20760558363813203No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT90440.20648684685178337No Hit
GCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCA90390.20637269003684983No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG89130.20349593830052468No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA85800.19589309442595101No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA83820.19137248455458294No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG83810.19134965319159622No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC80830.18454590702155738No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC80150.18299337433846125No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT79030.18043626168395002No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG77470.17687456905802362No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT76070.17367817823988455No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC75540.17246811600158907No Hit
GCTTAATGCTGGGCCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTT75460.1722854650976954No Hit
GTTCATTGATGCTTAATGCTGGGCCATTGCCATCATCCACTTCATTCTGA75100.17146353603017395No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT73740.16835847066398169No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT72580.16571003255752362No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC72050.1644999703192281No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG71950.16427165668936103No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA71040.16219400265757067No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT70020.15986520363292647No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA68310.15596104056219948No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT67720.15461399014598373No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC67520.15415736288624957No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG67300.153655072900542No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA66170.15107512888304406No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT66100.15091530934213712No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA65550.1496595843778682No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC65520.14959109028890805No Hit
GGATACACCATGGATATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGA63250.14440837089092545No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA62790.14335812819353688No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAA60890.13902016922606247No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA59740.1363945624825911No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCATCGTTTGTTTTGCTCCA58300.13310684621250518No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT58290.13308401484951848No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT57640.1315999762553825No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT57160.13050407083202054No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA54570.12459074781846326No Hit
GAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCA54320.12401996374379556No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT54100.12351767375808802No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGATATTCGAGAAAT53970.1232208660392608No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA53700.12260441923861971No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAACAGCAGACAAGAG53520.12219345470485896No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC53270.12162267063019128No Hit
CTTATCTCATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCT53240.12155417654123116No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC52400.11963634205034773No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA50420.11511573217897964No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT50380.11502440672703279No Hit
GAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGT49410.11280976451732215No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT49290.11253578816148167No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA49180.11228464316862788No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC48590.11093759275241215No Hit
GTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACAC46810.10687361014077819No Hit
AAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTT46590.10637132015507063No Hit
GTCTCTGGAATCATGTCCATTATTCTCTTGTCTGCTGTTTGATTATGGCC46360.10584619880637636No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG46210.10550372836157573No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT46180.1054352342726156No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA46180.1054352342726156No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACG4950.0121.93598145
AGTAGAA86600.0116.2977141
AGCGAAA13450.0113.209751
CGCTGCG458.185452E-11113.12134145
GCAGGTA18000.0106.7115252
TGACGCG1250.0104.71803145
CAGGTAC19000.0100.6906363
TTGCTCG4050.0100.55229145
AGATCGG2300.098.01506145
CGTATCG451.0697477E-896.96114145
CGGGCCG1800.092.9211145
ACGTAGG554.0745363E-1092.55381145
GGTACTG20850.091.405765
ACGACTG1050.090.03535145
TGCCGAG1050.090.03535145
GGTCAAA71800.089.170881
GAGCGAA1550.088.880961
CGAGTCG1400.088.303894145
ATTAGCG4550.087.90433145
TTCTACG502.222987E-887.26503145
Produced by FastQC (version 0.11.5)