FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524981
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG83900.23801546731741247No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA81160.23024237577450773No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA71970.20417131326381618No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC70190.19912164065565177No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC69710.1977599311882816No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT69250.19645495961538514No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT68390.19401523015301358No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT67600.19177408332130016No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG66620.18899392649208607No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC66030.1873201586051102No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT65810.18669604176589888No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA65550.18595844913774004No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC65130.18476695335379112No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT65020.18445489493418546No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC63800.18099388337128625No Hit
GAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTT62500.17730592023049202No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA62410.1770505997053601No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT62330.1768236481274651No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG59880.1698732560544298No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT59670.16927750816245535No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC58420.16573138975784552No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC55140.15642637506414928No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA54600.15489445191335785No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT54320.15410012139072524No Hit
GTCTAGAATTGACCTGCATGGTACCCGGGCATGCTCGAGCTAGCAGATCT53550.15191571245348556No Hit
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGA52970.15027031351374662No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT52820.1498447793051934No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG51670.14658235037295236No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA51360.14570291300860913No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT51090.1449369514332134No Hit
ATTCTAGACGGGCCGGCCCCTGCGTGTGGCACGGGCGGCCGGGAGGGCGT50700.14383056249097512No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT50250.14255395986531558No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA49920.1416177846064986No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG49810.1413057261868929No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT49620.14076671618939224No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT48150.13659648094557106No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA47830.13568867463399095No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT46870.13296525569925058No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA46680.13242624570174988No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC46520.13197234254595983No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT44980.1276035246714805No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG44770.12700777677950606No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG44610.12655387362371598No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG44480.12618507730963657No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG44080.12505031942016143No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG43860.1244262025809501No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG43840.12436946468647632No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT43750.12411414416134442No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG43740.12408577521410753No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA43660.1238588236362125No Hit
GTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAG43640.12380208574173876No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA43170.12246874522160545No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA42930.12178789048792037No Hit
GTTTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCC42820.12147583206831469No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT41900.11886588892252187No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT41600.11801482050541548No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC41560.11790134471646796No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT41300.11716375208830913No Hit
GTACCCGGGCATGCTCGAGCTAGCAGATCTTTTTCCCTCTGCCAAAAATT39970.11339068210580426No Hit
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG39870.11310699263343546No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA39780.11285167210830357No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT39660.11251124474146101No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC39510.11208571053290785No Hit
GTGTGTGGCTGCGATGGTGGCGTTTTTGGGGACAGGTGTCCGTGTCGCGC39340.11160343842988091No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA39230.11129138001027523No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA39140.11103605948514332No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC38730.1098729326484313No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG38550.10936229159816749No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA38490.10919207791474622No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC38190.10834100949763985No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT38060.10797221318356043No Hit
ATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGG38030.10788710634184978No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC38020.10785873739461291No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC37920.10757504792224411No Hit
CCCCAGGACACACGCGGGAGCAGCGCCGGGCCGGGGACGCCCTCCCGGCC37430.10618496950763708No Hit
CTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTG37320.1058729110880314No Hit
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG36830.10448283267342434No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT36670.10402892951763427No Hit
GCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA36500.10354665741460735No Hit
GCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG36430.1033480747839492No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG36250.10283743373368537No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT36130.10249700636684282No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC36070.10232679268342156No Hit
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTT36050.1022700547889478No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT35810.10158920005526272No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA35750.10141898637184145No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC35670.10119203479394641No Hit
CTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCT35550.10085160742710386No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA35380.10036933532407692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACGT100.007025163145.45734145
GCTTGCG550.0119.010544145
TCCCTTG256.3666976E-6116.36587145
TTTCGCG1100.0105.787155145
GGGATAG650.0100.70123145
GCATTCG607.2759576E-1296.97155145
TATCGCG301.5751968E-596.97155145
GTAGTCG301.5751968E-596.97155145
GTCGGCG554.0563464E-1092.56376145
CGGCCTA406.0070124E-790.910835145
GTCTCTG1550.089.151276145
GCATACG900.088.890594145
TCATACG259.272007E-487.2744145
AATCGCG502.2213499E-887.2744145
TCGCCGG1050.083.118484145
TGGCAAG1050.083.118484145
TGACACG353.3851928E-583.118484145
AGGATCG353.3851928E-583.118484145
ACTGTTG801.8189894E-1281.81975145
TCCGGTG554.3026375E-879.34036145
Produced by FastQC (version 0.11.5)