FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620170
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA715912.7323036291538334No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA348431.3297992115015438No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT348411.3297228805764512No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT231390.8831106378593755No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA216120.8248319765511398No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC215210.8213589194594244No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG196930.7515924539247454No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG185140.7065953735826301No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA163960.6257609239095173No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA157540.6012586969547777No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG150800.5755351751985559No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG137870.5261872321261598No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG135660.5177526649034223No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG131330.5012270196208644No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC123430.4710763042092688No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG119580.45638260112893436No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG117640.44897850139494766No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG115010.43894098474526466No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA110910.4232931451012721No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA105420.4023403061633406No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA103680.3956995156802803No Hit
GGATACACCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGG93260.35593110370701136No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT85900.32784132327291743No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC85740.32723067587217625No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC84680.32318513684226596No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT82920.31646801543411307No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG82830.31612452627119614No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA81770.31207898724128585No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA81000.309140246625219No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC80960.3089875847750337No Hit
CCATGGACACAGTAAACAGACACATTCCTGGATTCCCAAGAGGAATCGTT80370.30673582248480064No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG78030.29780510424896095No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA76060.29028650812733525No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG73690.2812412935038566No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT73410.28017266055255957No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC71990.27475316487098167No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC71020.2710511150039883No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT69380.26479197914639124No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA68390.26101359835430527No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT67210.2565100737738391No Hit
ACTTAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATGAA67130.2562047500734685No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG65940.2516630600304561No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA65460.2498311178282325No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT63000.24044241404183697No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC62830.23979360117854948No Hit
CTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTC60510.23093921386780247No Hit
GGATACACCATGGATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGA60390.23048122831724657No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATACTTCTGGTACATCTG60390.23048122831724657No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA60190.22971791906632014No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA59280.2262448619746047No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG59260.22616853104951207No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG58560.22349694867126943No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG57030.21765763290168194No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA55750.2127724536957526No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA55370.2113221661189923No Hit
ATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGG54630.20849792189056435No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT54500.20800177087746213No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATAGGAATCGTTCTA54110.20651331783815555No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAGCACCACATTC53700.2049485338737563No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC53570.2044523828606541No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT53280.2033455844468107No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT52700.20113198761912396No Hit
ACTTACAGTTCTTGGAAAGGATGCAGGTGCATTGACTGAAGATCCAGATG51580.19685745581393574No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA49180.18769774480281814No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT48700.1858658026005946No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTT48690.18582763713804828No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT46220.17640076788910644No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC45910.17521763855017042No Hit
CCTAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAA44870.17124843044535276No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG44630.17033245934424102No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC44460.16968364648095352No Hit
GAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAA43370.16552361106340427No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT42950.16392066163645871No Hit
GTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGA42930.16384433071136606No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT42190.16102008648293814No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT41940.16006594991928005No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA41550.15857749687997344No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG41150.1570508783781205No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT41070.15674555467774992No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA40770.1556005908013602No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA40590.1549136124755264No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA40060.15289084296057126No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGATACCC40040.1528145120354786No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG39920.15235652648492273No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATT39870.1521656991721911No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC39780.1518222100092742No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAA39360.15021926058232862No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTGCGCAAAATGCCA39260.14983760595686538No Hit
GACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGTGAGA38940.14861631115538304No Hit
GCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAGAACACAC38650.14750951274153967No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC38300.14617372155241837No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT38300.14617372155241837No Hit
TCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG38000.14502875767602866No Hit
ACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGCTAGA37960.14487609582584338No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT37780.14418911750000954No Hit
CCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCA37090.14155570058431324No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG36830.14056339855810882No Hit
GAACTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCC36610.13972375838208972No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA36490.13926577283153382No Hit
ATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTC35930.13712850692893971No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT35840.1367850177660228No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT35240.13449509001324342No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA35010.13361728437467799No Hit
GTTCTATTCTCAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATG34300.13090753653388904No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC34170.13041138552078682No Hit
GAACAGGAACAGGATACACCATGGACACAGTAAACAGACACATTCCTGGA33790.12896109794402652No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC33790.12896109794402652No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC33200.12670933565379344No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC32670.12468656613883833No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG32620.1244957388261067No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG32500.12403775327555083No Hit
CTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGT32370.12354160226244863No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC31990.12209131468568833No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG31900.12174782552277143No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG31750.12117534358457659No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT30730.11728246640485158No Hit
CTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATA30610.11682448085429571No Hit
GTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGACGGATCA30460.11625199891610086No Hit
CTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTGATCCTCTCACATTC30370.11590850975318397No Hit
GCCATGGAACAGGAACAGGATACACCATGGATTCGAGAAATTTTTCCCTA30170.11514520050225749No Hit
GAATGACCAGAACAGAGTAGGATGCAATTTTCCTCATTGACTGTGAATGT30000.11449638763896999No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA29600.11296976913711707No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA29510.11262627997420015No Hit
GAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTG29060.1109088341596156No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC29020.11075617230943031No Hit
ATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAG29000.11067984138433766No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA28810.10995469759595752No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA28660.10938221565776267No Hit
GTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGCCA28550.10896239556975312No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA28240.10777926623081709No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA28050.10705412244243695No Hit
GAACAGGATACACCATGGACACAGTAAACAGACACATTCCTGGATTCCCA28050.10705412244243695No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG28040.10701595697989062No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA28010.10690146059225164No Hit
GGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACAC27970.10674879874206634No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA27930.10659613689188106No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG27890.10644347504169577No Hit
GGGTTTGTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAGTGGAC27380.10449703645183327No Hit
GACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCC27190.10377189266345314No Hit
CTTCAATGGTGGAACAGATCTTATGATCTCAGAGAACTCTTCTTTCTTGA26940.10281775609979504No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT26790.1022452741616002No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA26780.10220710869905388No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT26650.10171095768595168No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC26480.10106214482266418No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC26320.100451497421923No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC26270.1002606701091914No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACG151.2149239E-4145.45645145
TGACGCG650.0123.07854145
CGTATCG256.366583E-6116.36516145
AGTAGAA56850.0114.0331
CCGCACG1950.0111.88958145
GCAGGTA12250.0111.2659452
CGCCGTG203.8187177E-4109.09234145
TTCTCGG2850.0107.178444145
ACGCTGG750.0106.66807145
CAGGTAC13400.0102.769663
CTGTTCG1500.0101.81952145
GGTCGCG501.9099389E-10101.81951145
GGTCAAA19050.095.163081
GGTACTG14750.093.347595
GAGCGAA1400.093.2326351
CCGACGG554.0563464E-1092.563194145
AGCGAAA7450.092.4678651
ACCGGAG1100.092.246833
ATACCGG1150.088.2782441
AGGTGCG502.221168E-887.27387145
Produced by FastQC (version 0.11.5)