FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_H02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_H02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082965
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC99120.3215086775230987No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT95560.3099613521399043No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC67240.2181017299904475No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG64850.2103494525562243No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC60630.19666133089412302No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT58770.1906281777444765No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC51580.16730647282729452No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT50030.16227884520258906No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG49080.15919739601325347No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA48980.15887303294069183No Hit
GTATAATTGGGATTAATTGTTCTATGAAGACAACCTGACAAACAACCCAA42490.13782186953144132No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA42100.13655685354845093No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG41480.13454580249856873No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA41100.13331322282283453No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG39700.12877213980697153No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT39090.12679352506434552No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA37280.12092255345097981No Hit
CTATTGGTGCTTCTTACATTCAGCAAGTGAAAGATGCAAATCCTAACCAT35670.11570030798273741No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA34420.11164576957571688No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT33050.10720199548162239No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC31980.10373131060521285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG608.112693E-1084.82358145
GGTCGCG1100.072.70592145
TAGATCG1350.064.62748145
GATCGCG1500.063.0118145
TGACGCG2600.053.13125145
TCGTTCG1258.731149E-1152.348263145
TCGCCGG1700.051.321827145
AGCGAAA8000.050.758651
GCATTAA32850.049.6661
GCGAAAG9000.046.72642
AGATGTA37100.043.367627
TGGTCGG2200.042.96259145
GTAGAAA30500.042.5564351
CCGACGG1806.91216E-1140.392178145
TAAGATG44400.039.990385
CGAAAGC10700.039.2930073
CATTAAG42200.038.486852
CGATCGG3250.038.03079145
CTATGCG2109.094947E-1237.961715
TTAAGAT44800.037.047434
Produced by FastQC (version 0.11.5)