FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072674
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC26320.12698571989613416No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT22590.10898964333030665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG300.001913125972.71788145
AGCGAAA3800.059.1348461
TGACGCG1205.820766E-1154.53841145
GATCGCG1101.6334525E-952.885723145
CGCATAG2100.045.015823145
CGAAAGC5500.040.838073
GGTCGCG907.353996E-540.39882145
TCGCATG1451.879198E-840.120205145
GCGAAAG5800.038.734172
CCGACGG1458.5024476E-735.105183145
GTAGAAA21500.034.7183722
TGTCTCG3450.031.616467145
AGTAGAA24050.031.0447061
GCATTAA16750.030.2934421
CCCTTAT14100.029.3036121
CGATCGG1000.005987512429.087149145
TAGAAAC30400.026.9321543
AACCTCG1100.00953346426.442862145
CAATTCG2507.8685844E-826.089979
CCTTATA16950.025.225682
Produced by FastQC (version 0.11.5)