FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701612
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC37480.10125318374805356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1650.057.300686145
CGTATCG953.0657247E-853.588898145
AGCGAAA7650.050.2244571
TGACGCG1901.8189894E-1242.105564145
ACGATAG1609.804353E-1040.909386145
TAAGGCG4000.040.000286145
GCGAAAG9850.039.006811
GTAGAAA27050.037.5198941
CGACGAG2807.2759576E-1231.169054145
ATTCGAC1902.0807238E-730.5236991
CGAAAGC13050.029.4383412
AGTACTG19650.028.7633295
GCATTAA25750.028.1529271
TAAGATG23550.027.9999715
CATTAAG27100.027.0146942
AGTAGAA26300.026.7372531
CAATTCG2808.247298E-925.882249
TAGTACT26400.025.5261964
GAGCGAA6100.024.9568791
TGTATCG1500.001467968524.242598145
Produced by FastQC (version 0.11.5)