FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763787
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA208930.7559555059778486No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG178060.6442609361719988No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA143820.5203729520400813No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC143430.5189618447441862No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT138350.5005812676591938No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT130450.4719972993577291No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG127420.46103408113577493No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA127140.46002097846179896No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG126480.45763295073028415No Hit
GTTCATTGATGCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAAT108960.394241669130074No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG104150.37683801248070126No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC103040.37282178402315375No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG98700.35711869257652634No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT96040.34749421717375467No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC93160.33707373252714484No Hit
GAATGAAGTGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAAT87290.31583475861200594No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT85780.310371240620207No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG84180.3045820824832015No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC83560.30233878370511186No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT82510.298539648677702No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT79280.28685278568862216No Hit
GCTTAATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCT78510.28406675333518827No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC77850.2816787256036735No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC76920.27831377743653907No Hit
TCTCAGGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAG74260.26868930203376745No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT71800.2597884713981215No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA71350.2581602706720887No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG67120.24285518384738045No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG63730.23058940504460004No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG60630.2193729111541519No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG60340.21832362624181964No Hit
GAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGG58430.21141281871576934No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG58330.2110509963322065No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG57720.20884387979247318No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG56230.20345272627738678No Hit
GTCTATATGCGTCTCCACATGGGGAAGATCCGAAAATCGAAACTAACAAG56040.20276526374861736No Hit
ACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCCTT55650.20135415645272228No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT55400.20044960049381522No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC54290.19643337203626762No Hit
CATTTAGACAATAACTTCTCAATAAAGCAAGATAGAAAATTGCTTCTCAT54200.1961077318910611No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC53270.19274278372392661No Hit
CAAGAGAATAATGGACATGATTCCAGAGAGGAATGAACAAGGACAAACCC53170.19238096134036378No Hit
ATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTG52840.1911869474746064No Hit
ATGCTGGGCCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAG52310.18926928884172334No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC51870.18767727035404683No Hit
GTTGTAATGAGGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCA51540.18648325648828945No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA51450.18615761634308287No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA51230.18536160709924462No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA50670.1833354017512927No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG50460.1825755747458107No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG50240.18177956550197247No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC49540.17924680881703256No Hit
GTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTTGTTTT48470.17537530931291015No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT47980.1736023796334522No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG47340.17128671637865003No Hit
GTTTTATTATCGTTTGTTTTGCTCCAGAGGGTTTGTCCTTGTTCATTCCT46750.16915196431562923No Hit
CTACTTTTCCAGCGCAAAATGCCATAAGCACCACATTCCCTTATACTGGA46360.16774085701973415No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA45750.16553374048000083No Hit
CTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTCCA45580.16491864242794396No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA45040.16296480155670462No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT44510.16104714292382155No Hit
ACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATCGTTT44220.1599978580114893No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG43680.15804401714024996No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA43290.15663290984435488No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC42890.1551856203101035No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG42720.15457052225804666No Hit
ATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGA42280.15297850377037014No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC42110.1523634057183133No Hit
GAATGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGAATAAT41820.15131412080598106No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT41740.15102466289913077No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT41650.15069902275392424No Hit
GAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGACCCAA41150.14888991083611003No Hit
CATTACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGG40730.14737025682514607No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC40630.14700843444158324No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT40430.14628478967445754No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT40410.14621242519774497No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC40200.14545259819226303No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC40120.14516314028541275No Hit
ATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTAT39640.14342639284431108No Hit
GAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGACTTACA39140.14161728092649686No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT39100.14147255197307174No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA38970.14100218287444005No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG38960.14096600063608375No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC38670.1399167157237515No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGATG38480.1392292531949821No Hit
CATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGT38270.13846942618950012No Hit
ATAATAAAACTTCTCCCCTTTGCTGCTGCCCCACCAGAACAGAGTAGGAT37790.13673267874839848No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA37430.13543011816757225No Hit
GTCTAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGATTTTCGGAT37310.13499593130729684No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA36130.13072642718125527No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA36070.13050933375111756No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA36060.13047315151276132No Hit
CAATTACAGCAGACAAGAGAATAATGGACATGATTCCAGAGAGGAATGAA35390.12804894154289026No Hit
GAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAAACGAC35360.12794039482782138No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT35270.12761475468261485No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC35080.12692729215384543No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA34850.1260951006716509No Hit
GTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTT34690.12551618485795035No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT34530.1249372690442498No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA34210.1237794374168487No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC33410.12088485834834595No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGCTTC33360.12070394715656453No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG33310.12052303596478312No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC33040.11954611552916343No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC32980.11932902209902572No Hit
CTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCAT32580.11788173256477436No Hit
CACCAGAACAGAGTAGGATGCAATTTTCCTCATTACAACAAGGCAACCAA31830.11516806468805302No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT31680.11462533111270876No Hit
CATCTGGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTGGGCAAA31550.11415496201407707No Hit
TGATTCCAGAGAGGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAAC31440.11375695739215792No Hit
GCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTG31410.11364841067708908No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA31110.11256294352640056No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA31090.11249057904968798No Hit
CTGTAAGTCGTTTGGTTGCCTTGTTGTAATGAGGAAAATTGCATCCTACT31060.11238203233461914No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG30790.11140511189899946No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGA30330.10974072893461038No Hit
CCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAATG30210.10930654207433498No Hit
GAATCAAATCGGAAACATGGCCAATCGGAGAGTCACCCAGGGGAGTGGAG30060.1087638084989907No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT29920.10825725716200271No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT29880.10811252820857759No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATGTCGTCCACTTTCCCT29410.10641196300583222No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT29220.10572450047706282No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA29090.10525413137843112No Hit
GAATGGATGATGGCAATGAGACCCAATTACAGCAGACAAGAGAATAATGG29040.1050732201866497No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT28660.10369829512911088No Hit
CTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGCAGACTC28400.10275755693184749No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA28370.10264901021677865No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT28240.10217864111814695No Hit
GAACAAGGACAAACCCTCTGGAGCAAAACAAACGATAATAAAACTTCTCC27960.10116553844417098No Hit
GAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACT27770.10047807591540159No Hit
TCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGT27730.10033334696197645No Hit
GGAATGAACAAGGACAAACCCTCTGGAGCAAAACAAACGATAATAAAACT27640.10000770681676989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG100.00702555145.45409145
TGACGCG650.0123.07654145
CGTATCG600.0121.21175145
CGAGTCG256.367156E-6116.36328145
AGTAGAA35200.0105.684341
GCAGGTA21550.0103.953022
CCGACGG1050.0103.89578145
AGCGAAA5350.0101.658431
CAGGTAC22700.098.6598743
AGATCGG1000.094.545166145
AACTGCG1200.090.908806145
GGTACTG25100.088.635915
TAAGGCG1000.087.27246145
ACGATAG1450.085.2662145
ACTGATC26850.082.594988
GTACTGA27850.082.227076
GCGAAAG7350.072.9911652
TACTGAT30650.072.826417
TCGCCGG903.6379788E-1272.72704145
CACACCG607.8647645E-872.72704145
Produced by FastQC (version 0.11.5)