FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_E04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_E04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAAGTTCATCTCTTTGGCCATCCGGAGACCATGGAGTAGGGCCTCGT22.8169014084507045No Hit
AAAAATAAATGAATAAAAATTATAATTTAAAACAAGCAATAAATCTAAAA11.4084507042253522No Hit
GGGTTGAGGATACTGGTAAGAGGCAATTCTCCAGTATTCAATTACAACAA11.4084507042253522No Hit
AAAGCAGGGGATGGCTCTGCGGCTGACCTGCAGATGACCTGCAGCGGCTG11.4084507042253522No Hit
AACAAGCTGTCGTGTTTGAAGTGAGCCTTCATGATTGCTGAATAACACAC11.4084507042253522No Hit
GTATTAACATGTCAGCGCTGTATTAACAAGTGAGCGGTGACATGTTTTTA11.4084507042253522No Hit
CCATTGTACCGGCCATTGTAGCATGCGTGAAGCCCAAGACATAAGGCTGT11.4084507042253522No Hit
ATCTTCCTGTATCAAGCAACAACAAGCACACCAGCATCCACTTCCATACT11.4084507042253522No Hit
TCAGAAACCAGGACTATTTTCCTAATTATGTTATCTTCACATATGGGCAG11.4084507042253522No Hit
AGACAAGAGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAG11.4084507042253522No Hit
TATAAAAATAATTTTTTAAAAACAACACAACACACCAAAAAGAAAAATAA11.4084507042253522No Hit
GACAAGATTTGAGAACAACTTATCAAAGGAAGCCACAAAACACTCATACA11.4084507042253522No Hit
GCCGCTTTGAAGTCAAATGTAGAAAAGGAAATATCTTCCTATAAAAACTA11.4084507042253522No Hit
TATTTTCCCCCTTCTTAAATAAAAAAAATAAATCATTAGGAATTAAGTAC11.4084507042253522No Hit
GCTCCGTGCGCTTCGAGGCCTCGGGGAGCTCTGTCCCTGGTGACGACGCC11.4084507042253522No Hit
AAAATGAAAAAAATTAATATAAATTCTAATAATAAAAAAATAAAATATAA11.4084507042253522No Hit
ATTTTATTCTTTATTCTTATTTATATTTAAAAAATATCACAAATAAACAG11.4084507042253522No Hit
GGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACATAACTCGAAC11.4084507042253522No Hit
TGAAAAATTATTATAAAAAATTAAACAAAACCCATCTACAGTTATAAGCT11.4084507042253522No Hit
AGTAGAAACAAGGTGAAAGAGGTGGGACGTGATATAAACCTATTTTGAAA11.4084507042253522No Hit
CACTACGTCCAATTGCACAATTAGCAAAGTAGCCTTGTCGCCATTTGAAC11.4084507042253522No Hit
CTCTCCCTCCATGTCTCAGCCACACTCCTGTCACACGTGCCATACATACA11.4084507042253522No Hit
GATGTGGCCTGGGTCATGTTTTCCTGGCGTATCCACTTGGGCCCGTGTGT11.4084507042253522No Hit
GTGCGGGACTGCGACATTAGATCTCTCAGTTCTTTTATTCTCTCCATATT11.4084507042253522No Hit
GACCAAACTCAAAGACACAACTAGAATCACACCGCCCCAAACACAACATA11.4084507042253522No Hit
CCTCCATTATTGTGCTCCCATGTCCCCTGCTGCTGTGCTTGCTCGCGCTC11.4084507042253522No Hit
CTAAAGCATGAAAAAAAAGGAAATAGGGAGGATTACATATATTCATTAAC11.4084507042253522No Hit
ACTACTACTAGTAGTAGTAGTAGTACTACTACTACTAGTAGTAGTAGTAG11.4084507042253522No Hit
GTTTATTTGTTTGTTTGTTTGAGATGAGTCTCGCTCTGTCACCCAGGCTG11.4084507042253522No Hit
GTAATGGGATGATGAGGGCACGGGAAAAAAATTTGTGTCACACTCATTCT11.4084507042253522No Hit
TAATTGAAGGGAAGATACACAACATGTTGCTGGAAACATAGGAAACCAGA11.4084507042253522No Hit
CAATAACACTCACATAAACACCATCCTATGTATAGCTCCGGAGGGAGTGA11.4084507042253522No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT11.4084507042253522No Hit
AAACAAGGGTCCAGTGTCCATCAAAGTCCAGGTGCCCAACATGCAGGATA11.4084507042253522No Hit
ACCCGTGTAGAGCCACCAGCAGCGCGTAGCAACCGGTGGCAAATATCAAC11.4084507042253522No Hit
GTAAATAGAGTAATCAGATTACTAGAAACAAAAAGTAGAATAGAGTTTTG11.4084507042253522No Hit
GACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAA11.4084507042253522No Hit
CCTATTATATATAGGGAAAAATGAAGAGCAAAATAAACGTATGATAACAC11.4084507042253522No Hit
ACACGATAGAAGGGGAATTCTTGAGGATGAACAGATGTACCAGAAGTGCT11.4084507042253522No Hit
ACCTGTCTGGGGCTGACTATCGCGCGCCGGGTCATCGAGGACGAGCGCGG11.4084507042253522No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA11.4084507042253522No Hit
TGATACTGATGTGGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGACC11.4084507042253522No Hit
ATAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAG11.4084507042253522No Hit
GAAATAACGTCGAAGTCCAACAATAGAGACATACACACAAATAAAACTCA11.4084507042253522No Hit
GTTTATTTACACTTCTTATTTCTTTGCTTCTTCAGTTATTTTTGGGAAGA11.4084507042253522No Hit
TATTTGTGCTACTAAATATTAAACTTTTGGCAAATTTTTGTTTAATATTG11.4084507042253522No Hit
CCATAAGACACACCCACCAGCATGCCATGTCAGTTTACCATTACCATGGC11.4084507042253522No Hit
GACTGAAAGGGGTCAAATTCAATTTTGTTGTATATCATTTTGTGATATGG11.4084507042253522No Hit
TGGGGGTATTGACGAACGTGTACGTCGGGTTGCCGGGGTCGATTCGCTCC11.4084507042253522No Hit
CACTAACTCCTTTTCATTCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCC11.4084507042253522No Hit
TGCTGATTCACAGCATCGGTCTCACAGACAGATGGCTACAACCACCAATC11.4084507042253522No Hit
GTCCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTT11.4084507042253522No Hit
CGTCCCAAGTAGAGCCGGCATCCCCTACCCTCTGTGAATATACTATTGGC11.4084507042253522No Hit
GGCTAATAGAGTTATGAAATGATATAATGGCATAAAGGACCAACACTGAA11.4084507042253522No Hit
ATATAAATCCTAATAACAACAAAAATTGATCTCATTATAGAACCATTAAA11.4084507042253522No Hit
GGGACGTAGTGTTGGTAATGAAACGAAAACGGGACTCTAGCATACTTACT11.4084507042253522No Hit
CTCTAAACCAGATTATATTGAGACAAGCCTATCATTTCTGCATCACTGTT11.4084507042253522No Hit
ATAGTAAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGA11.4084507042253522No Hit
TATTAAAAGGAATTACGACTGTCTCAATTTTACAGATGAATGGACTGAGA11.4084507042253522No Hit
GGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCAGTCATAAAGAGATTG11.4084507042253522No Hit
AAGTAGAAAAAATTAAAAATTAAATAGTAAACTATACAACCTGTCTCTTA11.4084507042253522No Hit
AGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCT11.4084507042253522No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11.4084507042253522No Hit
GGATGGCTCTGCGGCTGACCTGCAGATGACCTGCAGCGGCTGCCTCGGGC11.4084507042253522No Hit
GCCTGGTGTGACGGCATCCGTCATTCTTACGCGCTCATCACACCCACTTA11.4084507042253522No Hit
AGGCAGGCCCGGCCGGGGATCTGGGGCGGGGGCCGAGGGGCGGGGTGTGG11.4084507042253522No Hit
GACCGATAAAGGCCTTGATACAAACAACCTTGGATCAACACAAGAGCAGA11.4084507042253522No Hit
GTGTATGACTATCTGTTTGTACGCAAGCCATTTATACTTTATCTCCTCTG11.4084507042253522No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA11.4084507042253522No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC11.4084507042253522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATTGT50.0145.07
CTCCATT50.0145.02
TATTGTG50.0145.08
ATTATTG50.0145.06
CATTATT50.0145.05
CCTCCAT50.0145.01
ATTGTGC50.0145.09
ATTAAAG50.0145.0145
CCATTAT50.0145.04
TCCATTA50.0145.03
Produced by FastQC (version 0.11.5)