FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_E03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_E03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433315
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA68910.15543673300904629No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA68870.15534650707202172No Hit
CTTTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACT66020.14891790905902244No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT56590.1276471444054844No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG55070.12421855879855143No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG51390.11591777259229268No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG48200.10872225411458468No Hit
CATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAGGAAGGAAAG47230.10653427514173931No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC46410.10468464343273601No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC45640.10294779414501337No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC45450.10251922094414677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG404.7184585E-9109.08342145
AGCGAAA9900.061.515721
CGAAAGC12800.047.560313
GCGAAAG13200.046.1248172
CAATTCG17450.043.1944589
GGTCGCG1053.7474092E-641.55559145
TCGTTCG750.001461779638.785213145
GGTCGTT9200.036.236027
GTAGAAA34200.036.038351
CCGACGG2051.10703695E-831.926855145
GCTATTG34750.030.6586198
AGGTCGT11150.030.5477186
TAGAGCT42700.027.6612284
CTATTGA39400.027.4081769
TATAGTA20600.027.0927352
AGCTATC24750.026.9403219
TGACGCG1906.99436E-626.79242145
CCCTTAT20650.026.3319631
TGTATCG1958.557565E-626.105433145
GAGCTAT44450.025.1075766
Produced by FastQC (version 0.11.5)