FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4344623
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCCAGCTCAACCCG1138982.6215853481418296No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA635761.4633260469320353No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAA613291.411606944952416No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT479571.103824198325148No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT369430.8503154358847708No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGA312400.7190497311274189No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC291350.6705990370165605No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT281890.648824995862702No Hit
GGATAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA270800.6232991907468152No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG246510.5673910026255443No Hit
GCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGAT234670.5401389257479878No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG227770.524257225540628No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA206660.4756684296888361No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG205590.4732056153088542No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG197990.4557127281239362No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA190790.43914051921190866No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG176660.4066175592220545No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA174020.4005410826209777No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA172350.39669725083166013No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGA172120.39616786082474814No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT156190.3595018486068872No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG148880.34267645316981477No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG145760.33549516264126944No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA140990.3245160742370512No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC140930.3243779724961176No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA134730.3101074592663161No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG124020.285456298509675No Hit
TGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGA124010.2854332815528528No Hit
CCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAG121600.279886194958688No Hit
ATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCT120990.2784821605925301No Hit
ATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG119020.2739478200985448No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT117490.27042622570473895No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT115400.2656156817288865No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT113850.2620480534214361No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA108690.25017130370114965No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACA106770.24575204799127565No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG106610.2453837766821195No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT104570.24068831749037836No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA100130.2304687886612947No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC98980.2278218386267347No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG98350.2263717703469323No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG96930.22310336247817128No Hit
GCATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA93990.2163363771724267No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG93950.21624430934513764No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT92230.21228539277170885No Hit
GAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGA87300.2009380330583344No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC85300.1963346416938823No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG84270.19396389514118947No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT81780.18823267289244658No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT76350.1757344653379591No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT76290.17559636359702555No Hit
CCATGCTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAAT76020.17497490576282454No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC75610.17403121053311185No Hit
GCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGA74660.1718445996349971No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGC73080.16820792045707994No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC72050.1658371739043871No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC70660.16263781690609289No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA70410.16206239298553637No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG70380.1619933421150696No Hit
CCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATTCGA68560.15780425597341818No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC68480.1576201203188401No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA68350.1573208998801507No Hit
GGCCTCCACCATGCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGG67690.15580178072988152No Hit
TGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGT67470.15529540767979177No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA67450.15524937376614725No Hit
TCATATAGGAGACCGGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCG65040.14970228717198247No Hit
ACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGG64680.1488736767263811No Hit
AAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAG63840.1469402523533112No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC63580.14634181147593245No Hit
GAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTT63460.14606560799406532No Hit
ACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATT63030.1450758788507081No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG61540.14164635228419128No Hit
GAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATCG61420.14137014880232418No Hit
GCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGT58790.13531668915806966No Hit
GATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCAT56710.13052916213903945No Hit
CACAGAGACTGGTGCACCCCAGCTCAACCCGATTGATGGACCACTACCTG56500.13004580604577198No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA55670.12813539862952436No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA54720.1259487877314096No Hit
CTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCC54360.1251201772858082No Hit
TTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAA54150.12463682119254077No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA53900.12406139727198424No Hit
CAAACAGACTGTGTTCTGTAAACAATGCTGTGGTAATGCCAGCCCATGGT52960.12189780333069175No Hit
TGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAA52850.12164461680564688No Hit
GGTTAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGGATTGATGCCAGGG52680.12125332853966846No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC52550.12095410810097906No Hit
CTACCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCT52100.11991834504397736No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC51930.11952705677799892No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG51280.11803095458455198No Hit
ATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTT51140.11770871718904034No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT50910.11717932718212835No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG50300.11577529281597045No Hit
TATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCTGG50200.11554512324774785No Hit
CAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCA49740.11448634323392387No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA49440.11379583452925605No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT49170.113174376695055No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG47950.11036630796273923No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATAGAAGTGCTGCAATCTATT47940.11034329100591696No Hit
ATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGG47680.10974485012853818No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTTGCTGCAATATG46990.10815668010780222No Hit
GAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTGCACCCC46970.10811064619415771No Hit
AACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAG46640.10735108661902311No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTATGTTACGGCCA46380.10675264574164434No Hit
ATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCTCA46020.10592403529604294No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC45890.10562481485735355No Hit
GATCTTATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTA45610.10498034006633027No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG45490.10470413658446313No Hit
GCCATGGAACAGGAACAGGATAAAGTGGACGACAAACACAGAGACTGGTG45310.10428983136166245No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT45090.10378345831157272No Hit
GCTAGAAATTAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTCG44490.10240244090223707No Hit
GTTCTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTC44420.10224132220448126No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC44350.10208020350672543No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA43530.10019281304730007No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG43520.10016979609047783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGCG3350.0117.23582145
AGTAGAA81100.0115.324731
CGGGCCG1850.0102.2146145
TCTATCG451.0688382E-896.97284145
GCAGGTA9900.095.916812
TGACGCG700.093.509514145
GTAGAAA107950.086.6886442
AGCGAAA9000.085.391961
CGTATCG353.385062E-583.11957145
CAGGTAC11800.081.686113
TAGAAAC115450.081.5443343
CGACGAG1000.080.002594145
ATCATAG4200.079.65626145
GTCCACG651.5279511E-978.32421145
CCGACGG755.4569682E-1177.57826145
CAATGCG2850.074.005585145
GAGCGAA2450.073.9822541
CGCTGCG1000.072.72963145
ATCGTTA1400.072.72962145
GCGAAAG11000.069.850872
Produced by FastQC (version 0.11.5)