FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_D03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_D03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5000881
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT94190.18834681329149805No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA75460.15089341258070327No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT75260.15049348304828689No Hit
AAATAATAGGTCCTGGGTAGGGATAAATCTAATGCACCTTTTAAAATAGC66530.13303655895831154No Hit
CCATAGTGGTGTATGGTGACAGACGGTGGCTCCTTTTTTCCTGAGCACAG64080.1281374221862108No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA61700.12337826075045576No Hit
CTTATAGACTTGTCTAATCACTGTATTATACATCTGAAACTAATGTAACA61250.12247841930251889No Hit
GTATAACACAGCTTATCAGAGTTTTTTCTCTGCCCTCATGTGCTTGTTAT59930.11983888438857072No Hit
CCCTACACCTGCTTCAACAGGAGCGTGAAGAAAAGACACACACCAAACTG58410.11679941994220619No Hit
GTTGAATATATTGTGGACCTCTAGGTCGACAGCCCTGAAGCTATTTTAAA58320.1166194516526188No Hit
GCCCAGAACCAACCCACACACCACCACCTAAACAGGGCGGTTTCAAGCCA56650.11328004005694196No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC56190.11236020213238426No Hit
ATATTCAACTGTCTCTCCTTTGACCGATGTTCGAGTTATGTTTTGTTTTC55300.11058051571313135No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC52700.1053814317917183No Hit
CTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTATGATCCCACT52450.1048815198761978No Hit
ACCCAGGACCTATTATTTAGTGGGAAAAATGAAGTGCAAAATAAACGTAT52310.10460156920350633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA10900.055.866531
GCGAAAG14900.041.347482
GTAGAAA35700.038.1758351
CGAAAGC16400.037.118923
CAATCGC1051.8219685E-434.628807145
GCTATTG32000.032.3867728
CTATTGA34000.030.6948269
TGACGCG1453.421498E-530.091238145
TGTACCG2951.4551915E-1129.4788445
GAGCTAT37400.028.8724236
GCATTAA32050.028.4997771
CAATAGG47450.027.6529371
GTCTAGT25650.027.1320631
ACCGTGC36350.026.9160258
TACGCGG1958.559484E-626.104792145
CGCACAA10050.026.049133145
CGACGAA3151.0004442E-925.394459145
ATAGGAG54500.024.9989193
TAGAAAC55550.024.3991072
GTACCGC3001.7176717E-824.157246
Produced by FastQC (version 0.11.5)