FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_D02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_D02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602852
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG145400.315891104037236No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA132940.28882093102276585No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA111120.2414155397566552No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG95010.2064155006504663No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC88700.19270660885902915No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC82410.1790411683886425No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG78270.17004674493118613No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC74250.16131302940003286No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT72850.15827143692649687No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT71130.15453462331615267No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG67310.14623542099550452No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG65310.14189028889045316No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT61150.13285241411194623No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC60710.13189648504883494No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA59060.12831175106216755No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG53310.1158194962601448No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT52530.11412489473917475No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA47030.10217578145028344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16900.063.5000041
CAATTCG51150.049.7290429
GCGAAAG22450.048.4398572
GCAGGTA30950.046.614432
CGAAAGC23350.046.5591283
ACTGATC33000.046.5549438
CAGGTAC32250.045.171743
GGTACTG34150.044.3501135
GTACTGA45900.043.7317436
CGTATCG855.2469175E-542.78047145
GTAGAAA53600.041.5309941
AGGTCGT31350.040.4510886
GGTCGTT31050.040.142217
GCTAAAC24750.040.136931
GAGCGAA11150.038.3685951
GGTCAAA47350.037.365311
AGTAGAA54100.036.322091
AAGGTCG38100.034.4272359
TACTGAT47400.032.4109657
TATATTC70000.031.6828213
Produced by FastQC (version 0.11.5)