FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3700591
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA46110.12460171902271826No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA41430.11195509041663887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6850.055.0467571
CGTATCG450.00952644148.480026145
CGTTTCG901.3276695E-648.316869
GCGAAAG11750.038.879591
CGAAAGC12700.035.9630052
CAATTCG7450.031.1303259
TAGTTCG1000.00598724129.088018145
GGTCGCG1504.311835E-529.088017145
TATACGT7100.028.5821914
GTAGAAA27700.027.225291
TGACGCG2705.3969416E-926.933348145
AGTACTG23600.026.1023315
GTATACG7300.025.8166073
GACCGTC3451.09139364E-1025.2091397
ATAGACG1500.001498358624.1574525
TAGTACT30700.024.0800654
CAATAGG34300.023.6778831
GGTCGTT7050.023.6447417
GCTATTG27800.023.4632248
CTATTGA28900.022.570169
Produced by FastQC (version 0.11.5)