FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_C03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_C03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094680
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA83350.2035568103001944No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG68720.16782752254144404No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA67320.16440845194252054No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC60170.14694676995516134No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA57740.14101224027274414No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC50900.124307638203718No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT47760.11663915128898962No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC47050.11490519405667843No Hit
GTGTAATTTTTATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCC46820.11434348960114099No Hit
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA43410.10601561049947737No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA43290.10572254730528392No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC41060.10027645627985582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG502.2648746E-672.727974145
AGCGAAA9400.048.5954931
ACGAACG803.6680438E-545.454987145
CGAAAGC12150.037.5772253
TCAATCG1051.8208098E-434.63237145
TCGTTCG900.003574155732.323544145
GGTCGCG900.003574155732.323544145
GCGAAAG14400.031.7131422
TAGATCG1453.4189463E-530.094334145
CTATTGA32350.029.1246919
TCCGACG2252.8265276E-828.9948752
GCTATTG32450.028.8115928
GGAGCTA34050.028.5199077
GATTAGC12400.028.0674441
AGCTATC36000.026.5744139
TAGCGCA1100.00969662226.3515724
GTAGAAA29200.026.3212381
ATAGGAG53900.026.2184053
GCATTAA26400.025.8170871
CAATAGG53850.025.0443941
Produced by FastQC (version 0.11.5)