FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3963039
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC151270.3817020221098001No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG121370.3062548715770902No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG98010.24731020815086605No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTTCTCACATA76610.19331124422444493No Hit
CTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCATGGC57090.1440561145121206No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC54760.13817678806592618No Hit
GTGGTAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTCGTCA50760.12808352378061383No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG44330.11185860144197421No Hit
GATTTGAGATAATTGAAGGAAGAGACCGAATCATGGCCTGGACAGTGGTA42040.10608020763863286No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT41640.10507088121010165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC33500.073.782573
GCAGGTA33600.073.362072
ACTGATC39700.063.5242778
GGTACTG39500.063.1144525
GTACTGA43500.060.3088846
AGCGAAA8050.056.754811
TGACGCG953.0642695E-853.592155145
TACTGAT51700.048.640217
GACAGTA39050.046.3954627
CGAAAGC10650.044.239273
TTGTCCG1652.7284841E-1144.08013145
GCGAAAG10550.043.2932322
GTAGAAA29550.041.2296681
AGGTACT63800.040.0994034
AGCTATC14450.039.1181039
GTCGAAC750.001485492938.6583183
CTGATCC72900.036.1846929
TATCCCG1905.2677933E-934.4521145
GGATAGC47850.034.3804365
ACAGTAT62500.033.2776688
Produced by FastQC (version 0.11.5)