FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_B02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_B02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4790936
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT85110.17764795856175078No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC79950.16687762057351632No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC64240.13408653340391105No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG61310.12797081822842135No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC57280.11955910076861806No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG55110.11502971444410863No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT54960.11471662322352041No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG52320.10920621774116791No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC52250.10906010850489341No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA51990.10851741705587385No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA49740.10382104874705068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG553.6379788E-12105.77194145
TGACGCG1100.099.161194145
TCGTTCG406.012506E-790.89776145
CGACGCG400.00606146754.3541566
GGTCGCG901.2974833E-648.478806145
CGAAGCG1850.047.168564145
AGCGAAA9900.043.9441151
CGAAAGC10850.040.0759663
GCATTAA33700.039.373671
CCCTTAT28900.038.386481
GCGAAAG12050.036.0959242
CCGACGG2052.8376235E-1035.472294145
TATCGCG1251.2402899E-534.90474145
TTATACT38000.034.5178574
AACGACG850.00270289834.220333145
CATTAAG40900.033.1445582
AGCTATC28600.032.689889
CGATTCG900.003576567932.319202145
GATTGCG1652.3335833E-630.85015145
CCTTATA36300.030.7544572
Produced by FastQC (version 0.11.5)