FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S6_R1_A04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S6_R1_A04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362227
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT62050.1845502995484838No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC60710.18056484585960436No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC34990.10406792878648585No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC34800.1035028271440328No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT34620.10296746769328782No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC34060.1013019049576367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA8250.052.7280161
CGAAAGC9800.045.104023
GCGAAAG10200.043.3442572
TGACGCG1201.9273466E-742.42423145
TATACTG27350.037.3559955
GGTCGCG1301.6208482E-533.56642145
TTAGGTA15150.033.4798854
TGTATCG1352.0983101E-532.323223145
GCTATAT7800.031.6030061
TAGTACT15950.030.8920544
CCGACGG1504.3088054E-529.090899145
GACGACG2303.3882316E-828.458488145
ATTCGAC1358.08604E-426.8522281
TTATACT23400.025.391984
GTATTAT38100.025.3087561
GTAGAAA20800.024.7479461
CCCTTAT18850.024.231111
CCTTATA18550.024.2242152
CTAGTAC18750.022.4149723
ATATACT31450.022.1181684
Produced by FastQC (version 0.11.5)