FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_H11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_H11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244427
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC59220.2638535358913433No Hit
GTAATATACTGTGCTGCTTCTGAGAAACCAGCAGGGCCTGTTAATTCTTT51940.2314176402262136No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT44990.20045205301843189No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC38640.17215975391491903No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT38180.17011023303497955No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC33830.1507288942790298No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG32740.1458724208896079No Hit
GATAACATACTAACGTATGCTTCCTAGAATGAGAAGGAAGAAAATGAATA31880.14204070794015577No Hit
CCATAATAAATAGGAAACACTCGAGGTTCACTACAATTTGTCCTTATTCC30510.13593670010207506No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG27540.12270392398594386No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA26990.120253409890364No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG26760.11922864945039424No Hit
GTATTATCTCACATGCAAAAAATATCTCAGTAAGAAAAAATGCAACCCAC26550.11829299861390014No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC26420.11771378619130851No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT26140.11646625174264967No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT25530.11374840883664294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4600.074.074011
TGCGCCG406.562001E-572.72824145
TATACTG14650.062.3254055
GCGAAAG6600.051.619282
GTAGAAA16150.044.4414831
ATATACT20100.044.3407064
GCTATAT5550.043.1069031
CGAAAGC8250.042.1627733
AATATAC20600.041.1585923
AGATGTA20800.040.763757
CATTAAG24200.038.0405162
AAGATGT23150.037.8779076
TAATATA23300.036.398882
ATACACG1406.4537016E-736.36412145
TGGTCGG1655.8582373E-835.26218145
AAATGCG1905.2696123E-934.450222145
GTAGTAC2750.034.2572563
TGACGCG850.002700763334.225056145
CTATATA7600.033.382012
TACTGTG39050.033.033197
Produced by FastQC (version 0.11.5)