FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_H10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_H10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701976
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATATGGAAGGCAAGAGAGACAAGGGGGAGAGATGGAGGGAAAGAGC35440.5048605650335625No Hit
AGTATATACTGAGTATTTCGGGTTATCTAATCTTAAGAATGTGATTTAGC27370.3898993697790238No Hit
TTGTAATACAGTCTGAAGTCAGGAAGTGTGATACCTTCAACTTTATTCTT26730.3807822489657766No Hit
ACTCTATACCAACCAAACCACCTATATCAATATTCTGATGACCTTTTTCA16060.22878275040742135No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA15100.21510706918755057No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC14850.21154569386987587No Hit
ACACTAAGGGATTCCAAAATTAAAGATTTTAGTGAGTTAGGTTAAGTGAC13460.1917444471036047No Hit
ATATTGGTCTTGGCAATAATTTTTTTGGATATGACACCCAAACCACAAGC13350.19017744196382783No Hit
GATATGTACAGTATATATGGAAGGCAAGAGAGACAAGGGGGAGAGATGGA12910.18390942140472039No Hit
CTTATATACAGAAAATCCTAAAGACCACCAAAAAACTGTTAGAGCTAGTC12330.17564703066771514No Hit
TCAATATTCTGATGACCTTTTTCATAGAAGTAGCAGAAACAACCCTAAAA11410.1625411694986723No Hit
GTATTACAAAGCTATATTAATCAAAACAGTGTGGTACTGCCATAAAAAAT11250.16026188929536053No Hit
ACCTATATCAATATTCTGATGACCTTTTTCATAGAAGTAGCAGAAACAAC10600.15100231346940637No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC10210.14544656797383385No Hit
CCTTAAATACTTTGGATATTAACTCCTTATCTGACAGATGATTGATAAAT10090.14373710782135002No Hit
ATCTTAGGGGGAGAAAGGCTGCTGCCCGTGCAGGTCTGGGTCCTGTCCAT9610.13689926721141463No Hit
GTGTAAAAATAGGCATCCATTTCATTCTTCTGCATGTGATTATGCAGGTT9570.1363294471605867No Hit
ATATATATCTCCTCTTAAAAGAGCCTGAAATCTATTAGTTTCCTATTTAT9480.1350473520462238No Hit
TCTCTAGGCTAACCTGTGAATGCTAATTTACTTTATCTTAGGGATCCTGC9440.13447753199539586No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC9430.13433507698268887No Hit
ATCTTAGGGATCCTGCCTTTCAGATGATTACAGTTACCAAGGAAACAGGC9380.13362280191915393No Hit
GTACCACACTGTTTTGATTAATATAGCTTTGTAATACAGTCTGAAGTCAG9080.12934915153794432No Hit
GGTTTATTTCAGGGCTCTCGCTTCTATTTCTTTGGACTATTTTTTATGGC9070.12920669652523734No Hit
CACCAATAGCATCAAAAAACAAAAAACTTGGGAATAAATTTAACCAAGAA9050.12892178649982336No Hit
CCTTATAACAGTCTCCTCCAGGAATGATTTCTTGAATATATACCAAGGGA8650.12322358599154387No Hit
GCCCTATTCACCAATAGCATCAAAAAACAAAAAACTTGGGAATAAATTTA8510.12122921581364605No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA8240.11738293047055741No Hit
GTCTCATACTCACGACAACCATTTACCAAACTCCGTTATGTTTTTGTATG8230.11724047545785041No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG8140.11595838034348753No Hit
TGCATGCACTGGTTTATTTCAGGGCTCTCGCTTCTATTTCTTTGGACTAT7720.10997526980979407No Hit
CATTAAACCTGTTTTGATTAAATACTAGTGGTGATTGTGGCATTTGACCC7720.10997526980979407No Hit
GTCTAACATTAGCTGACCATGCATGCACTGGTTTATTTCAGGGCTCTCGC7720.10997526980979407No Hit
GAATTATTTGATATTGGTCTTGGCAATAATTTTTTTGGATATGACACCCA7590.10812335464460324No Hit
GTACAGTATATATGGAAGGCAAGAGAGACAAGGGGGAGAGATGGAGGGAA7550.10755353459377529No Hit
GTGTGGTACTGCCATAAAAAATAGTCCAAAGAAATAGAAGCGAGAGCCCT7400.10541670940317047No Hit
GTTATAAGGTAAAAATGAAAGTAGAATAACACTTCACAAAAAAACCCAGA7350.10470443433963554No Hit
GTTTTGATTAATATAGCTTTGTAATACAGTCTGAAGTCAGGAAGTGTGAT7280.10370724925068663No Hit
CTCATTAGGTTAAAGCTCTTTGAAGGTAAAGGCCTATTTTCTAGTTCTTG7250.10327988421256566No Hit
CTTACTAGAGAGTTCAGAAGCACAACTTGGGGGTATACCTTTTGCAGAAT7210.10271006416173772No Hit
ATATATACCAAGGGACCTTCATTCCGGTTAATTCCTCCTAGGATCTTCAG7180.10228269912361677No Hit
CCCATGAAAGACCCAAAATAGCCTTTGTTCTTGAGAAAGAATAAAGTTGA7080.1008581489965469No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA7060.10057323897113292No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCTA100.007014737145.52133145
CGTATCG100.007014737145.52133145
CGCTGTA100.007014737145.52133145
AGTATTG100.007014737145.52133145
AGTTACG202.0924417E-6145.52133145
TGCGTTA151.2123904E-4145.52133145
CTGACGG600.0121.26778145
AGCGAAA2200.0118.553731
GTTTTCG203.8107688E-4109.14099145
CTGCCGG451.0642907E-897.01423145
CGACGAG301.5708742E-597.01422145
GCGAAAG2900.089.918082
CCCATCG259.2526927E-487.3128145
CCGTCAG259.414251E-486.9332059
ACTATTG300.001908136172.760666145
ACCGTCA300.001941406972.4443368
CGTTTCA406.6872E-572.4443368
ATGGTCG406.689562E-572.439173
ATACTGA6850.070.857966
CCTAAGC1350.069.756243
Produced by FastQC (version 0.11.5)