FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_H06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_H06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022442
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4200.060.4313961
GCGAAAG4800.054.3696252
TTGACGT450.00965415248.314374
CGAAAGC6150.041.2439733
CTAGTAC2450.038.4542923
TAGTACT5850.029.731924
AAGTTCG1000.005983581829.08966145
ATAGTGT3102.910383E-1128.0548766
TATACTG4000.027.178165
GTATAAT4550.027.0945171
TATAACG1100.00969020126.3532924
GTCCTAT6550.024.357081
ACTGTGC12250.024.2581588
CTAGGTT5700.024.1571854
GTCCTAG8250.022.8540551
TATGATG4455.456968E-1222.8011535
AAGTGCG1600.002178910222.6495769
GTATATA6450.022.48611
AATAGTC3554.141839E-922.4570735
TAGGTTG6450.021.3492535
Produced by FastQC (version 0.11.5)