FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686499
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT31730.4622002362712837No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC25180.36678858964106287No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG21970.3200295994604508No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA20170.2938096049666496No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA17460.25433394658987124No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14880.21675195448208956No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC14220.20713795650102915No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT14090.20524429023203236No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG13610.19825229170035208No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT13050.19009496008005838No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT12510.18222896173191802No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG12490.1819376284597647No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT12300.1791699623743079No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA11800.17188663057047424No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT11750.1711582973900909No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT11380.16576863185525398No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC11370.1656229652191773No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT11280.16431196549448723No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT11240.16372929895018057No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC11130.16212696595333714No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC10570.15396963433304345No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT10260.1494539686146666No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA9840.1433359698994463No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG9830.14319030326336965No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA9700.1412966369943729No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC9530.13882030418106947No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC9440.1375093044563794No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA9330.135906971459536No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA9250.1347416383709226No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG9230.13445030509876926No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC9020.13139130574115912No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT8900.12964330610823904No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA8850.1289149729278557No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT8800.12818663974747233No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA8750.12745830656708895No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC8570.12483630711770885No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC8520.12410797393732546No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT8420.12265130757655875No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG8360.1217773077600987No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC8270.12046630803540864No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT8100.1179899752221052No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC8050.11726164204172185No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG7980.11624197558918513No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG7950.1158049756809551No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC7940.11565930904487844No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG7860.11449397595626504No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA7820.11391130941195836No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA7640.11128930996257824No Hit
CTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT7630.11114364332650156No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC7540.1098326436018115No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT7480.10895864378535147No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG7470.10881297714927479No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC7450.10852164387712146No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA7180.10458864470305129No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA7160.10429731143089793No Hit
GTAATGGACAGGCCTCATACAAGATCTTCAGAATAGAAAAGGGAAAGATA7160.10429731143089793No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG7020.10225797852582452No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT6990.10182097861759448No Hit
CTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT6970.10152964534544114No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC6960.10138397870936447No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT6930.10094697880113446No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC6910.10065564552898111No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTAG100.0070175026145.5019145
ACTATGG100.0070175026145.5019145
CAAACGG100.0070175026145.5019145
TACGGAG100.0070175026145.5019145
GGACACG100.0070175026145.5019145
TATCGCG202.0937987E-6145.5019145
GTAGTCG100.0070175026145.5019145
AAGTCCG100.0070175026145.5019145
GTGTGCG151.21302415E-4145.50189145
CTCGCCG151.21302415E-4145.50189145
AGGCTCG351.8571882E-9124.71591145
TCGCGCG256.3533153E-6116.40153145
CACCTAC256.4767555E-6115.95193
CCACCTA256.479102E-6115.943462
CTCGCCC203.8127563E-4109.126434145
CCACGAA203.8127563E-4109.126434145
CGCCACG203.8127563E-4109.126434145
AGCGAAA2000.0108.681151
GAGCGAA600.0108.681141
AAGCGAG301.5718917E-597.00127145
Produced by FastQC (version 0.11.5)