FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456783
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC10990.2405956438834195No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG7610.16659989535512487No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC7560.16550528369050513No Hit
CTACTAACCTGTTTTTGAACTCAGAAATAAATCTACTCCCTCTTTTGTGT6270.13726430274331575No Hit
TTCTAGTACTGTGCCACTATTGAATTGAATAAGTTCATTGTTGTTTAAAT6240.13660753574454393No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT6220.136169691078696No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT5640.123472195769107No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA5630.12325327343618304No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG5620.12303435110325908No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC5100.11165038979121376No Hit
GTACTAGAAATGCTGAAGCAAAAGTCATGACTACTCCTTAAGAATGATGG4890.10705302079981084No Hit
ATTTTATAGAGGTGGAAACCGAGGCGCAGAAAAGTGAAACAACTGACTTG4880.1068340984668869No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTG203.8154738E-4109.10093145
GCCGTAG301.5731484E-596.9786145
CGGTCCG259.2641084E-487.28075145
TGGTCGG300.001910488172.73395145
TTATCGC300.001939815772.4552153
GCGAAAG1206.002665E-1154.3473631
GCTATAT1351.0284566E-842.9411281
GTCCATA907.498478E-540.257311
CATTAAG4000.038.038992
CTATATA1553.4820914E-837.396242
GCTGTAC1001.393531E-436.2355428
GTAGAAA3400.036.227612
CGAAAGC1604.6053174E-836.2276082
AGTACTG4250.035.8092465
AGTAGAA3500.035.196391
TATTATC1707.849485E-834.0965732
TAAGATG4100.033.5841645
GCTTAGT3750.032.8499641
CTTAGTT3950.031.1832582
TCTATGC3750.030.9219
Produced by FastQC (version 0.11.5)