FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584213
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCA33910.5804389837268257No Hit
GTGTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAG24340.416628866526421No Hit
GTACAGGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCA20750.35517867627046984No Hit
GTCTAATATCTGAAGAATGACAGATTCACGCACATATACACAGAATGTGG20360.348503028861049No Hit
GTGTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAAAG20300.34747600618267654No Hit
AAGTAAATCTGACCAAGAAAATTGACTTACTTGCTATGGTTACTTAATAC19660.33652109761337046No Hit
GTGTATCATTGTACTTAAAATAGCCATTGGGATAGGGGAAAAGGGAAGCA17620.30160232654870744No Hit
GTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCT17370.2973230653888222No Hit
GTGTAACTTCTTCCTTCCCTGAGCCTCCATTTCCATGGGTGTAAAGTGAA17260.29544019047847275No Hit
GGTGTAAAGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGGATTTTGCAT16310.27917899807090907No Hit
CCTTAGGGAAGGGATTGGCCACCGGCATCCGGGCATCCCGCAGCTCTTCG16050.2747285664646285No Hit
GTCCAGGGAAAGTGGCCGGAGCGGGGGTAGGGCTGGTGGCCACCATGGTT14970.25624215825392455No Hit
TATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACA12800.21909817138612117No Hit
GAACATGCATTGTGTAACTTCTTCCTTCCCTGAGCCTCCATTTCCATGGG12780.21875583049333036No Hit
CTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAA11340.1941072862123917No Hit
GTATACTCCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCT10970.18777397969576165No Hit
CTTAATACACTTGGTACAGTGCCTGGCATACAGCTGATAATCAATAAATG10790.18469291166064433No Hit
CTGGTGGACAGAGGACCTTCCGAGAGACTCGGGCGTACGTAGCCACATTT10170.17408034398412908No Hit
GGGCTATACCTTCGTCATTCCCTCAGGCTGGATTCATGCTGTGTATACTC10130.17339566219854743No Hit
GGATAAGAGGCCGGCGGGGTGGACAGGCCGCTGTACAGGCTGGGGGCGAA9920.16980108282424392No Hit
TCCCAAGCCACAGCCCTCATGTCTGGCTGACCAAGCACAAATGTGGCTAC9500.1626119240756368No Hit
GATGAAATAAGGGCTCTGAAAGCTTTACATTGTCTAATATCTGAAGAATG9470.16209841273645056No Hit
GCTTCAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACA9400.16090021961168272No Hit
TCCCAGGACCGTGGGATCATGACCTGAGCCGAAGGCAGAGGCTTTAACCT9320.15953085604051948No Hit
GCCATGATGTCCTGGGGCATCAGCATCCGGCTGTTCAGAAACTCTTCACG9200.15747681068377456No Hit
ACACTGGGGATCCCACACTTGGCCGTTATGGGAGGCCCGGCTAGAGTCAG9190.15730564023737917No Hit
GTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTGACTGCAAGAATT8850.15148584505993534No Hit
CTCCACCACCTCCCGACCCCGATAAGCCATGATGTCCTGGGGCATCAGCA8810.15080116327435372No Hit
CACATATACACAGAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGG8730.14943179970319045No Hit
GTGTCCCAGCTTGTTAGATTTCTAGCATAAAAGCCTTGTGACTGCAAGAA8700.14891828836400423No Hit
CATTAGTCTTTGGAGGCAATTTTTTGCATAGCTTCAATATCCCCATGCAG8700.14891828836400423No Hit
ATATTAGACAATGTAAAGCTTTCAGAGCCCTTATTTCATCCATGCAAAAT8660.1482336065784226No Hit
GAATGTGGCTTAAACATGGGATCATAGGTCTAAGCGGGCACATGTTGTGT8400.143783174972142No Hit
CCTCTAGGCAGCTTGCAGGGTGAGTCCTTTCTCCCACTGGGCACCGGTTC8390.1436120045257466No Hit
GTATGGATTTGGAGTTAAATGGGTTGGAATCTGGAAATGGGGATGAAGAA8370.1432696636329558No Hit
GTTCAGAAACTCTTCACGGGGAGGCTTGAGGGCAGGCACCATCTCTGCTT8060.13796337979469817No Hit
TCCCTAGACTTATTGTGGTGATCGTTTTGCAGTGGATACAGATACCAAAT8050.13779220934830277No Hit
CCTACAGACACATTAGTCTTTGGAGGCAATTTTTTGCATAGCTTCAATAT7910.13539582309876705No Hit
GCTTTACATTGTCTAATATCTGAAGAATGACAGATTCACGCACATATACA7890.13505348220597624No Hit
GTGTGAGGCTTTGTGATGGGGATGGTAGGAACCCGCACCTTTGGCCGGGC7830.1340264595276038No Hit
GTCCACCAGTTCTCACTCGGAGGCCCGTGTCTCCTCTCTGTTTTCTCCTG7830.1340264595276038No Hit
GGTCTAAGCGGGCACATGTTGTGTGGCTGACTCGCTGCTCTTGTCTGGGC7800.13351294818841758No Hit
GCCCCATACTGGCTGCCCACGGGGCCTGACGACGTCTCTAGGAAACTGGC7580.12974719836771864No Hit
GGCTGGGGGCGAAGGCTCTGCTTTCACACCCGGGGGCGTTCCTCAGCGGC7570.12957602792132322No Hit
CCGTAGTGCATGTCACTGCGTGTCTCCTCTGGGGCCGCCTCCTTGGGGGA7560.1294048574749278No Hit
GCATAACAAACCGCTCCCACCTAACTAAGGAATTTCAAAAAGAGTCCCTC7550.1292336870285324No Hit
GTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAAAGCT7530.12889134613574157No Hit
GTATGGGGCACTCCGGGCCGAGGGGGCAGCAGGCTTGGGGCCTTCGGCCA7430.12717964167178752No Hit
GGTCTGGCTGCTGCCTGTCCTCCAAAGAAAGCTGCCTCCAAGCCTCTAGG7400.1266661303326013No Hit
GTATCATTGTACTTAAAATAGCCATTGGGATAGGGGAAAAGGGAAGCAAT7120.1218733578335299No Hit
CAATATCCCCATGCAGTTAAAAATATACAACATTGAAGATCGAACACGGG7100.12153101694073908No Hit
CACCGGGAGAGATCTCCATGGCTGGACGGCTGTAGTCACCGGGGATAGGC6930.11862111935201716No Hit
ACATTGAAGATCGAACACGGGTTCCAAATAAGTTCCGTTATCCATTCTAC6830.11690941488806308No Hit
GTGTTGGAGCGCTATGTGTACTGCATAACAAACCGCTCCCACCTAACTAA6700.11468419908492279No Hit
AATCCATACTGAGGGACTCTTTTTGAAATTCCTTAGTTAGGTGGGAGCGG6590.11280132417457332No Hit
CTGTGTATATGTGCGTGAATCTGTCATTCTTCAGATATTAGACAATGTAA6580.1126301537281779No Hit
CTTCTTGTGCAGTGGCAGAGACTCCAACTTATCTACAAGACAGCGGAGGC6570.1124589832817825No Hit
GCATCCGGCTGTTCAGAAACTCTTCACGGGGAGGCTTGAGGGCAGGCACC6480.11091844926422384No Hit
CCTCTTAAGTAAATCTGACCAAGAAAATTGACTTACTTGCTATGGTTACT6380.10920674480026976No Hit
GGCTTGAGGGCAGGCACCATCTCTGCTTCACTGCAGGAGAAAACAGAGAG6370.10903557435387434No Hit
CTCTTAAGTAAATCTGACCAAGAAAATTGACTTACTTGCTATGGTTACTT6350.10869323346108355No Hit
CATTTGTGCTTGGTCAGCCAGACATGAGGGCTGTGGCTTGGGAAGAGGGA6310.10800855167550191No Hit
ACATGTGCCCGCTTAGACCTATGATCCCATGTTTAAGCCACATTCTGTGT6180.1057833358723616No Hit
GTCTAAGCGGGCACATGTTGTGTGGCTGACTCGCTGCTCTTGTCTGGGCC6120.10475631319398918No Hit
CTACAAGACAGCGGAGGCCTTCAAGCTCAAAATGGGTCAGATGCACCTGC5970.10218875649805807No Hit
GGATACAGATACCAAATCATTACGGTAGACACCAGAACTCATAGAAGGTT5940.10167524515887186No Hit
AGTGAGAACTGGTGGACAGAGGACCTTCCGAGAGACTCGGGCGTACGTAG5900.10099056337329022No Hit
CCCCAGGACATCATGGCTTATCGGGGTCGGGAGGTGGTGGAGAGTAACCT5860.1003058815877086No Hit
TTCCATGGGTGTAAAGTGAAGACAGTAATGCCTTTCTTCCCTTGCAGGAT5850.10013471114131318No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG100.007018081145.49605145
AATTTCG151.21312645E-4145.49605145
CGAGTCG100.007018081145.49605145
TATTGCG100.007018081145.49605145
AGAGTCG100.007018081145.49605145
CCGTATA100.007018081145.49605145
AAGTTCG202.0939533E-6145.49605145
CGAATTG100.007018081145.49605145
AGCGAAA2100.0120.789871
GCTGTCG256.3537864E-6116.396835145
TTACACG203.8130785E-4109.12203145
GGGCTAT1550.0102.866221
GTAGCCC301.5720081E-596.99737145
GCGAAAG2850.088.987832
CGAAAGC2900.087.461043
CTCTCCG259.2582987E-487.29762145
GGCTATA1850.086.170452
CTATACC1950.078.0354844
GCTATAC2100.075.9185643
TCGCCGG300.001909290872.748024145
Produced by FastQC (version 0.11.5)