FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499513
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15360.10243325666399691No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3900.072.5051041
CGAATTG657.228531E-444.75143145
CGAAAGC6700.043.2693023
GCGAAAG7100.038.797782
TCGCCGG1102.3935566E-433.055035145
CAATTCG5300.031.4518859
CTATATC10700.029.798284
TTCTATA12350.029.348422
TACCTAT5400.026.8412067
GTATTAC8400.025.8946781
TCTATAT13450.025.865073
TATATCT14000.024.32725
TATATAC20700.024.1586933
TATAATG7300.023.8325252
TAATACC8050.023.4045224
GTATAGG4353.6379788E-1223.3349761
GTATTAA8450.023.167311
GTATATG5950.023.1528911
CAGCTCG10300.022.5168429
TATACAG12600.022.4293335
Produced by FastQC (version 0.11.5)