FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861937
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC11950.13864122319844724No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT11680.13550874367848229No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC9250.10731642799879806No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC8930.10360385967883964No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG259.266271E-487.28231145
CCGTATA350.003520854762.3445145
AGCGAAA2100.041.4374921
GCATTAA7150.039.5539741
CGAAAGC2200.039.533313
GCGAAAG2451.8189894E-1235.5096052
CATTAAG8700.032.4994542
GTACACC5450.030.5869353
GTTTCGG950.00473807430.5116339
GCTATTG5100.029.836858
TAAGATG11600.029.3625745
CTATTGA5000.028.9860489
GTCTAGT3403.6379788E-1227.726561
AGATGTA11350.026.175317
GATTAGC2001.0697093E-525.3613228
AACAAGG14750.025.0673732
GTATATA5500.025.0508521
ACCGTGC5550.024.806448
TAGTACC2951.43372745E-824.5658744
CGCACAA2101.5162177E-524.245083145
Produced by FastQC (version 0.11.5)