FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312363
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT57600.4389029559656894No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC39590.3016695837965563No Hit
TAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAGGC31650.24116803049156368No Hit
CCCTAATACTCATGCTGTCTTCAACCAAACTTCCAAAATTTTAACAAAGG29460.2244805743532849No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA25560.19476318670977466No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC25380.1933916149723819No Hit
GTACAGTGGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTC25050.19087706678716176No Hit
ACCTAAGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATAT23020.17540878552656544No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA22220.16931291113815308No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG21820.1662649739439469No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA21000.16001670269582424No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT20410.15552099533437014No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC20230.15414942359697736No Hit
GGTTATGTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGG19740.1504157005340748No Hit
CTTTTAGACTAGGAAGGAAGGGAAGTACAGTGGTTATGTATTTGGATGTA19730.15033950210421965No Hit
CCATTACACAAATCATTTGGCTGGGAATGGAACATTTACCATATGTATGA19240.14660577904131708No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG19240.14660577904131708No Hit
GTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATACT18160.1383763486169604No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT17810.13570940357202999No Hit
ATATTGCACATTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGA17460.1330424585270996No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC17080.1301469181926037No Hit
GTATTTACATGTGTAATCACCTTCCAGATCATGATATAGAATACTTAAGT16870.12854675116564548No Hit
TTTCAAACATGAATCCGTAGACCATTTCCTCCACCTAAGACATAATAACA16240.12374625008477075No Hit
CATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAAAAG16230.1236700516549156No Hit
GGTGTATTCCTTCCCCATCTTGGGAATTCTAGTTAGTCGAAGAGCAATAC15590.11879335214418571No Hit
GTGCCACACAGCCCCGCTAGAGTCTCAAAAGGCACTTTGACCTAATGTCC14230.10843036568388473No Hit
GATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAAC14070.10721119080620224No Hit
GTCTGGGAGAGCCTAAACGCCCGGAGCCCAGCAGGTGCTTTAACGCGAGA14040.10698259551663677No Hit
GTATTTGGATGTATTTTATAAAGAAGCCCCTAAATTCCGTATGGGAGCTT13240.10088672112822444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2050.077.796791
TCGTTCG300.001911893872.72818145
GCATTAA13750.071.7016451
CATTAAG16950.058.572632
GCGAAAG2850.055.9399032
TAAGATG21100.049.109375
ACGCACG450.00952086748.48545145
TATCCCG752.5040657E-548.48545145
TTAAGAT25250.041.0347984
AGATGTA22700.040.8612337
CGAAAGC3950.040.3570863
ATCAGCG1458.48986E-735.110153145
GAGCGAA1251.2662882E-534.7963831
AAATGCG1051.8201051E-434.632465145
CCATCGG3150.034.50979
AGTAGAA12200.034.463631
ATGAACG1905.455149E-934.325456
GTAGAAA12250.034.3229681
ACCATCG3500.033.129318
ATTAAGA32450.032.1545223
Produced by FastQC (version 0.11.5)