FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171882
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC14310.12211127058867702No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC11990.10231405551070841No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG300.001909709772.74864145
CGAAAGC4100.038.9025082
GCATTAA7350.038.4858861
GCGAAAG4200.037.992481
AGCGAAA3450.035.7399641
CGAATTG850.002696528334.234653145
GTAGAAA10250.032.550541
AAATGCG1453.4102548E-530.102888145
GATTAGC3400.029.8657461
CTAAGCG1255.168505E-428.9864374
CATTAAG9400.028.5372852
AGTAGAA11050.027.568381
ACCGTGC5600.027.1736248
TAGTACT5950.025.576274
ATGAACG2301.1074826E-625.2066736
TGTCTCG1450.001202328425.08574145
GGCTTAT10450.024.2927111
GCTATTG5550.023.5015138
TAGCACA5600.023.2926714
GTATACC1902.2004412E-422.884034
Produced by FastQC (version 0.11.5)