FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658253
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA14030.2131399325183478No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC8320.1263951702460908No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC7720.11728013393026693No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG7660.11636863029868454No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC7410.11257069850042462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2400.063.4294473
TAAGACC2750.060.610244
AGCGAAA2650.057.48051
GCGAAAG3000.050.73972
ACGAATG450.00950711648.50048145
ACCATCG3050.040.386298
CATTCCG750.001458065838.800385145
TACGACC850.002747104334.1029474
GTAGAAA5600.033.676921
CCGAACG1102.4435951E-432.9353458
CCATCGG3700.031.3330849
CGAACGT1255.1695545E-428.98319
TTAAGAC6050.028.7566833
ACCTCTC5850.028.4876638
CGGTTTT2053.9155202E-728.390526145
ATAGTAC1306.485381E-427.8810793
GATAATA2603.7234713E-927.878962
TAGTACT3153.45608E-1127.6071514
TATACTT6900.027.3029215
TGAACGT2156.150476E-726.9610237
Produced by FastQC (version 0.11.5)