FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107685
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG52280.47197533594839686No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT46590.420606941504128No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT35740.32265490640389644No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA35640.32175212267025377No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT32330.2918699810866808No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC29770.268758717505428No Hit
GGCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAA29000.2618072827563793No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG28840.260362828782551No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA28260.2551266831274234No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG24970.225425098290579No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTTTGT24530.22145284986255118No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC24380.22009867426208712No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT23880.21558475559387372No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA23250.2098972180719248No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG23250.2098972180719248No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT20680.18669567611730772No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT20550.18552205726357224No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT20290.18317481955610124No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG20270.1829942628093727No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA19670.17757756040751657No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA19520.17622338480705255No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG18970.17125807427201778No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG18310.16529970162997604No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA18030.1627719071757765No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT18010.16259135042904796No Hit
CTTATTAGATAGCCTCTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGT17890.16150800994867676No Hit
GTCCTATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATC17760.16033439109494127No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA17620.15907049386784147No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA17490.157896875014106No Hit
CTTCAGGAATGTGAAGATTCCGTATATTGTATAAGTTTGGTCCTCCATCT17430.1573552047739204No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT17190.15518852381317794No Hit
ACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGA17090.15428574007953524No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT16880.1523898942388856No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG16830.15193850237206427No Hit
ATATAAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTAT16780.1514871105052429No Hit
CTTTTTAACTTGCCTCTCTCTGCATCTTTGGTCATCGTATTTAATGTCAG16530.1492301511711362No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA16510.14904959442440768No Hit
ATCTGATACTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCT15800.14263982991554458No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT15760.1422787164220875No Hit
CAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCT15420.13920925172770238No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG15340.13848702474078822No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA15320.13830646799405968No Hit
CTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAATCCATAGCGA15200.13722312751368845No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA15080.13613978703331725No Hit
GAATAGAACGATTCCTCTTGGGAATCCAGGAATGTGTAGTTGCAACGGCA14980.13523700329967453No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT14970.13514672492631027No Hit
CTATTGTTCTTTGCGTGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC14930.1347856114328532No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG14840.1339731060725748No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT14650.1322578169786537No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA14600.13180642511183233No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT14560.13144531161837528No Hit
CTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT13960.12602860921651912No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT13930.12575777409642633No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA13620.122959144522134No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA13480.12169524729503424No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT13390.12088274193475582No Hit
CATTATGGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTC13260.11970912308102033No Hit
GAGTAAGAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGG13250.11961884470765606No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT13230.11943828796092754No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT13150.11871606097401337No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA13130.11853550422728483No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG13070.11799383398709921No Hit
GCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTAATGGAATC13030.11763272049364214No Hit
GCATTGTTTACAGAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGG12870.11618826651981384No Hit
CTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCA12300.11104239923805054No Hit
ATGTAATGGAATCAATGAACAAAGAGGAAATAGAGATAACAACCCACTTT12150.1096882236375865No Hit
GGACAACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGA11970.10806321291702967No Hit
GTTCAAGCTTTTCGCAAATGCTCCTAGCTAAAGTTTCAACAAAGTATACG11930.10770209942357258No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG11930.10770209942357258No Hit
GTCTTGGGAGTCTCGATACTGAATCTTGGACAAAAGAAATACACCAAGAC11810.10661875894320136No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT11760.10616736707638001No Hit
GGCCAACACCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGT11730.1058965319562872No Hit
GAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGACCTGCA11550.10427152123573037No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA11490.10372985099554476No Hit
CTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGC11390.10282706726190208No Hit
GACAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCA11320.10219511864835219No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG11310.10210484027498794No Hit
GAGGAAATAGAGATAACAACCCACTTTCAAAGAAAAAGGAGAGTAAGAGA11240.10147289166143804No Hit
ATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT11230.10138261328807377No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG100.007024721145.4559145
CGTCTCG100.007024721145.4559145
CGACACG100.007024721145.4559145
CGCTGCG151.2147536E-4145.4559145
TCCTATG100.007024721145.4559145
CTGAACG202.097675E-6145.4559145
TTATACG151.2147536E-4145.4559145
GTGTAGG100.007024721145.4559145
ACGCTGG100.007024721145.4559145
GGCTGCG100.007024721145.4559145
GAGCGAA100.0070944317144.976291
TACCGAT100.0070973067144.956633
CGGACCT100.007104017144.910835
GTACCCG256.3650587E-6116.364716145
TACATCG256.3650587E-6116.364716145
AACTGCG256.3650587E-6116.364716145
AGTAGAA3850.0109.202931
AGATCGG203.818184E-4109.09193145
CTCAACG203.818184E-4109.09193145
TGACGCG203.818184E-4109.09193145
Produced by FastQC (version 0.11.5)