FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157138
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA80870.37489488386927494No Hit
CCTATCAGGTGATCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCAC43790.2030004570871219No Hit
GTTTAAGACCTCTCCACAAAAGACAATCAACTTGCTCACACCTTATATAC36770.17045733745360753No Hit
GTATAATTCTTTGTACAGAATCAGGAAGACGGCATCGTTGCCAACCTGTG34410.15951691546855137No Hit
GGCTTATGAACGTCAGTATGAACAGCAGACCTATCAGGTGATCCCTGAAG32570.15098709493782966No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC30990.14366257513427513No Hit
AGTATGAACAGCAGACCTATCAGGTGATCCCTGAAGTGATCAAAAACTTT30070.13939766486891428No Hit
ATATGTGCCTGTAGTATAATTCTTTGTACAGAATCAGGAAGACGGCATCG28260.13100691749901955No Hit
GAGCAGGACCATCGGCATTAAGAATGTAGTTGAAGAGATTGCAGTAGTTG25430.11788768266100731No Hit
GTGTATTCTTGAAGAACCTTTCAGTCAACTTGGTCCAGCTGTTCTCATAG24540.11376184555647344No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT21750.10082804159956386No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA21690.10054989527790989No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG100.0070279837145.4365145
GGTCGCG607.870403E-872.718254145
TGACGCG809.640644E-1172.71825145
AGCGAAA4800.069.489491
CGTATCG501.9811135E-458.1746145
GCGAAAG5750.057.989782
CGAAAGC5750.057.980373
ATGAACG12100.046.7143026
CCGACGG1109.733594E-846.27525145
TAAGACC22300.038.6725584
GAACGTC14800.038.1956378
TTAAGAC23500.037.317153
AACGTCA16000.035.3309679
ACGAATG1458.50232E-735.105362145
TGAACGT16350.034.5730447
ATAGTTA4550.033.4478847
CCATCGG12100.032.343679
ACCATCG12200.031.4845078
GTTTAAG23100.030.762141
GCTTATG18400.030.728282
Produced by FastQC (version 0.11.5)