FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_F10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_F10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944008
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT50160.2580236295323887No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA47880.24629528273546197No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT31490.16198493010316828No Hit
GTGCAGGTACAACGGGATCCACATGCCCAGGGCTCCTGTGGCGAAGGACA29540.15195410718474409No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA24850.12782869206299563No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC24250.1247422850111728No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA22610.11630610573619038No Hit
GGGTTAATACACCAGTGTGTTTACCAATAATGAGGACACAGGGACTTTTT21190.10900160904687634No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6950.0121.029671
TGACGCG550.0118.979996145
TTATACG203.8223775E-4109.064995145
AAAGCCG203.8223775E-4109.064995145
ACGGTCG203.8223775E-4109.064995145
AGATGCG451.0704753E-896.94666145
CCGACGG700.093.48428145
GCGAAAG9800.085.066052
AATCCCG300.001913930272.71145
GAGTTAG1500.072.466837
CCTCTCG651.3710269E-767.11692145
GTATTCG350.003526370762.322853145
CGCTCAA350.003526370762.322853145
GTTAGCC1850.058.7568869
AGTTAGC1900.057.210658
AACTGCG651.0754458E-555.930763145
GACCGTC1051.1150405E-955.2128227
AAGGTCG400.0059828954.532497145
AGCCGAT1351.9099389E-1048.3099756
CGAAAGC18400.046.4768753