FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_F08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_F08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661758
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC27100.16308030411166968No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC18480.11120752841268103No Hit
GTATTATACATCTGAAACTAATGTAACATTGTGTGTCCACTATCATCAAT17530.10549069118367416No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT17450.10500927331175777No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC17430.1048889188437787No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT17330.10428714650388324No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT17280.10398626033393552No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT16890.10163934820834322No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA16830.10127828480440593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3550.067.388551
GCGAAAG4150.057.6386832
CGAAAGC4650.051.433233
CACATCG2050.046.11744145
TAGATCG907.3499185E-540.40203145
GTAGAAA13250.038.298581
AATGGCG1050.00759486627.70425145
CTATGCG2705.6006684E-926.8374124
GCATTAA9100.026.2889391
ATGAACG2808.249117E-925.8797116
AGTAGAA17150.025.3622421
CTATACG5150.025.3376181
GCTATTG6950.025.0240158
ACCGTGC5500.023.715948
ATACGGG2202.238725E-523.056476
GTCTAGT4455.456968E-1222.8070181
CTTAGTG7650.022.7342369
GAGCTAT7750.022.4402166
TAGAAAC22900.022.1569882
GCCCCGA6250.022.0294748
Produced by FastQC (version 0.11.5)