FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_F07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_F07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154926
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC23740.2055542952535487No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA15770.13654554490937082No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT14820.12831990967386656No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC14710.12736746769922921No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT14460.12520282684778072No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA14030.12147964458328932No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC13970.12096013077894169No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA12600.10909789891300395No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG12100.10476861721010697No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT12020.10407593213764343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG451.2107485E-680.80267145
AGCGAAA2750.073.8232961
TAGATCG406.563259E-572.722404145
GCGAAAG3800.053.41552
AGCGCCG604.8712586E-448.4816145
CGAAAGC4650.042.081463
GTAGAAA10950.039.0666351
TCGCCGG951.0105954E-438.274956145
TCAAGCG800.002006349936.361202145
ATGAACG3050.035.642776
GAACGTC3550.032.6627588
AGTAGAA10750.032.3743361
TGTCTCG950.00465728630.619965145
TAGAAAC14850.029.7780482
TGAACGT3900.029.7314837
TTTTCCG1606.688089E-527.270903145
ACCGTGC3750.027.0556498
CTGAAGT13650.026.5448174
CTTACGA1100.009677093526.3608952
TAGACGG1100.00969140426.3529055
Produced by FastQC (version 0.11.5)