FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_F04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_F04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269980
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT89790.707019008173357No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT61410.48355092206176475No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA43650.3437062000976393No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG36760.28945337721853887No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG35200.2771697192081765No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA33540.2640986472227909No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG33470.26354745744027464No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT32470.25567331769004237No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA31780.25024016126238213No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA30900.24331091828217766No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT27170.21394037701381124No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC25280.19905825288587223No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT24290.19126285453314226No Hit
GTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCT23960.1886643884155656No Hit
TCCCACAACAGGCCCCCAGCAGAGCCAGGAAGGGAGCCAGCAGTGGACTC23470.18480605993795177No Hit
AAGCAGGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTG23330.18370368037291926No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA22610.178034299752752No Hit
GCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAAC21930.17267988472259405No Hit
CCCTTGCACTTTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCC21610.17016016000251974No Hit
GCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTC20830.16401833099733854No Hit
GTACATGTCTGGCACCTGATAAGAGCTGATAACTGTAGATGGGTTTTGTT20390.16055370950723633No Hit
GCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCAAGGGGTGCTT20360.16031748531472936No Hit
CATCTACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTT19310.15204963857698547No Hit
CCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGG19260.15165593158947385No Hit
CCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGG19210.15126222460196223No Hit
GGTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGC19130.15063229342194365No Hit
CTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAG18680.1470889305343391No Hit
CCCTAAATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCC18610.14653774075182288No Hit
GTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCTGGAAGCCAGAACCAAG18530.14590780957180427No Hit
CCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAGCAGAGACAGAGG18250.14370305044173923No Hit
GTGCAAGGGACTGCAGGGGGTTTGTGGTCCTCAGAGCTTTGCGGGGGAAG17710.1394510149766138No Hit
CCCTTTACAGGAAATGTGACAAAAATCTTCAGAATTGCTGAAATCGCTGA17450.13740373864155342No Hit
GGTTTACATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAA17290.13614387628151622No Hit
GATAAGAGCTGATAACTGTAGATGGGTTTTGTTTAATTTTTTAAAAGCCT16910.13315170317642797No Hit
GTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGAT16880.132915478983921No Hit
GTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCC16790.13220680640640012No Hit
CTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTGATGTCATT16710.1315768752263815No Hit
ACTTACCACTTTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCC16170.12732483976125608No Hit
GGACATGCCTTTGCCAGGAGAACTGTTAGCCACTTCCGCCTCCAGTCTCA16160.12724609836375375No Hit
GGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAACAGGCCCCCAG16140.1270886155687491No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC16070.12653742578623287No Hit
GCTCTGAGGACCACAAACCCCCTGCAGTCCCTTGCACTTTAGAATGCAGG16010.12606497740121891No Hit
GTCCAAATCCCTCATTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGG15940.12551378761870266No Hit
CCTATTGCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTA15850.12480511504118176No Hit
GCCCAAGGCACCATGCTGCTAGGAAAAAGGCTTTTAAAAAATTAAACAAA15850.12480511504118176No Hit
TTATGTGCCTGATGTCATTCAATCTTCTCAGCCACCCTGAGGAACAGGTA15790.12433266665616781No Hit
CATAAAGTGCTTTGTACATGTCTGGCACCTGATAAGAGCTGATAACTGTA15580.12267909730861903No Hit
ATAAAAAGTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAA15290.12039559678105166No Hit
ATGTAAACCTCCCTCCCTCCCTCCTTTTCCTGCTTTAGAGTGCCCTAATG15180.11952944140852613No Hit
GTCTCAGTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCT15070.11866328603600057No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA14960.11779713066347502No Hit
TTGCACAGCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGAT14770.11630104411093087No Hit
CGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGGCA14680.11559237153340998No Hit
ACAGTTATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGT14480.11401754358336352No Hit
GTCCATTCATCTGTAAAATGGAGACAGTCGTAATACCTGTTCCTCAGGGT14280.11244271563331706No Hit
GACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCAAACGACTTGGT13990.1101592151057497No Hit
TATCAGCTCTTATCAGGTGCCAGACATGTACAAAGCACTTTATGTGCCTG13670.10763949038567536No Hit
GGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAA13640.1074032661931684No Hit
AAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCA13560.10677333501314983No Hit
GCAGTGGACTCTGGATCCAGTGCTCCCCCTCAGCCACCCCTTACTCAGAG13430.1057496968456196No Hit
CCCTCAGCCACCCCTTACTCAGAGAAGGAAACTGAGGCTGGAGGCGACAT13390.10543473125561031No Hit
CTGTAGATGGGTTTTGTTTAATTTTTTAAAAGCCTTTTTCCTAGCAGCAT13240.10425361029307548No Hit
TTAGAATGCAGGGAAGCGGCTTCTCTCCGTCCACCGCCCCCACCCGCCTA13020.10252129954802437No Hit
GTACTGGCACTTCCCACCCTCCCCTGCCTGGACTGGACATGCCTTTGCCA12920.10173388557300116No Hit
CCTCAGAGCTTTGCGGGGGAAGCACCCCTTGGAAGCTGGGACCAGGGGCT12860.10126143718798722No Hit
GAGAAGGAAACTGAGGCTGGAGGCGACATGAAGCTCTGGGAAAGCTGGGT12710.10008031622545235No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG301.5744967E-596.97539145
TGACGCG259.269172E-487.277855145
ATCGCTA651.5261321E-978.32627145
TACTACG300.00191152972.73154145
AGCGAAA2550.056.8308941
ATATACG400.006066577554.340264
GTAGAAA10150.052.113651
GACAGTG28500.051.1009257
CAAGGAC27950.049.7733653
GGACAGT30050.049.427646
GCCGACC604.8684276E-448.487694145
AACAAGG30450.047.5923731
ACAAGGA29400.046.829342
GCGAAAG4300.043.8126561
CGAAAGC4450.042.337492
CAGTGGG34100.041.6498579
AAGGACA35500.038.982124
AGGACAG38050.038.8450785
GGGCTGA17000.037.0821571
ACAGTGG41600.036.753698
Produced by FastQC (version 0.11.5)