FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_F02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_F02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249015
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT24220.19391280328899171No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA17500.14011040700071656No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT15040.12041488693090155No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC13950.11168801015199976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA353.3881173E-583.099754145
AGCGAAA3350.071.446211
GCGAAAG4750.050.3762742
CGAAAGC5500.044.8108023
GGACAGT20500.037.8291746
GACAGTG20350.037.7533767
GTAGAAA12600.037.415611
GATATCG950.00466060530.615696145
CAATTCG2951.4551915E-1129.484319
TACCCGA1000.006084675428.9905826
TAGAAAC16850.028.4020522
ATTCGAC2104.9708797E-727.6299921
GAACGTC3850.026.3571878
ATCGCTG15200.026.310364145
CAAGGGT8200.025.633974
AGTAGAA14100.025.2050191
GGGTACG1450.00120588325.0732145
CCATCGG4350.024.9938839
GAGCGAA2051.286642E-524.7659071
TGAACGT4101.8189894E-1224.7490487
Produced by FastQC (version 0.11.5)