FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_E10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_E10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639974
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11050.1726632644451181No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT9430.14734973608302837No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA7030.10984821258363622No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG406.54425E-572.759964145
TACATCG350.003573990262.107396
AGCGAAA1800.060.424681
CGAAAGC2550.042.622723
GTATTAC1901.2914825E-1038.1629561
TTACGTA850.00269355734.239983145
TAGACGG850.002748933934.0981754
CATACTA1551.4704565E-632.723255
TATACTT4050.032.203835
ATATATA5400.030.874072
GTATATA8350.030.3932551
TATATGT5150.029.5462344
GTATGGG2252.8101567E-829.0038471
TATATAC9250.028.983453
CTAATAC1753.788622E-628.983453
TACTATA1555.5041164E-528.0594642
CTATTCG1050.007724961327.6054429
ATATACT5550.027.4167774
CTAAGCC2405.2947144E-827.1719843
GTCTAAG1358.073249E-426.8554151
Produced by FastQC (version 0.11.5)