FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272433
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG19370.15222805444373103No Hit
GTATGAAAAGATCTCCAGAAGGAGTGCTCGATCTTGCCCACGCTCTTGAT16170.12707938256866963No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT15920.12511464257843047No Hit
CTTCTATATCTCAAATAAGTCTGAAAAGAAATTTGCTTTTCTCTCAGGTG14570.11450504663113893No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA14280.11222594824246149No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG151.2160349E-4145.41869145
CGAAAGC5900.067.5747153
AGCGAAA6350.062.818071
CTGATCG350.003526214762.3223145
GACGATG501.9820014E-458.167477145
CTTATCG400.005982626754.53201145
GCGAAAG7350.054.2607232
ATACTGC2450.050.28686
CAAGTCG450.00953054848.472893145
GTCGCAT1255.2168616E-946.3821758
TATCGCC657.3427014E-444.6087653
ATATATC2600.041.8108566
GTATAGG1105.2523246E-639.559811
GTTCTAT1901.2914825E-1038.171751
TCGCATC1553.4833647E-837.4049849
CATGACG800.00200819736.354675145
GGTATCG800.00203319836.2631571
CGTTTCG1001.3948102E-436.236089
ACGCAGA800.002040667536.2360766
ACCCGTG6050.034.7387168
Produced by FastQC (version 0.11.5)