FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_E08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_E08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319328
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG16570.1255942419170972No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT15900.12051589900312887No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC13230.10027832351015062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4100.056.5864641
GCGAAAG5250.045.561942
GTAGAAA10500.044.1913341
CGAAAGC5550.041.7820053
ACCGTGC7800.031.5887958
AAATGCG1606.683791E-527.273905145
TAGAAAC20050.024.2218882
ATAGGAG9750.023.7836023
CCGTGCT11200.023.2934179
GTCTAGT5000.023.200451
AGCTATC7500.023.1898929
GCATTAA7700.022.597841
CCAATTG1650.002553805722.039518145
GCTATTG7900.022.015728
ATAACCG2003.1145156E-421.7388745
CACCGTG11400.021.6117487
TCCACCG11450.021.5173725
GGACAGT10900.021.272716
GAAACAA21000.021.0479554
CACATCG2805.927637E-620.780117145
Produced by FastQC (version 0.11.5)