FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_E07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_E07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341124
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC17590.13115864006609382No Hit
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA13650.1017802977204196No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC13640.1017057333997453No Hit
GTATAATAAGGATATGTAATGCAACAACATTTTTAGTGAAAACAAAACAT13490.1005872685896308No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA451.17562806E-464.64676145
AGCGAAA3900.063.233191
GCGAAAG4150.059.4128762
TGACGCG501.9795819E-458.182083145
CGAAAGC5350.047.4297263
GTAGAAA12700.047.4029271
GCCGACC907.3468065E-540.40422145
GGCTACG750.001486316338.65217
AGTAGAA10650.032.6905631
TAGAAAC19300.030.8110352
GCATTAA7850.029.567261
ACCATCG3500.028.9901568
CCATCGG3403.6379788E-1227.712219
CATACCG1050.00772356227.6086435
AAATGCG1357.937477E-426.93615145
CACATCG1658.2430204E-526.446402145
ATACTCG1100.00952606926.4464145
ACCGTGC6500.025.6451388
GTATAGG3851.6370905E-1124.4913861
GAAACAA23550.024.3132464
Produced by FastQC (version 0.11.5)