FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_E06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_E06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904086
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG9740.10773311388518349No Hit
GTTCAATGGCTGAGCCAAGCCTTGAATGGCATGTCAGGTAGGCCAGGAAA9140.10109657709554179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2400.066.471421
CGGTTCG1251.8189894E-1258.0114251
CGAAAGC2900.054.9743163
GCGAAAG3000.053.1565442
GGTTCGC1600.049.834262
TGACGCG700.001040127741.560974145
ATAGTAC3700.039.1708873
TCGCAGT2002.2555469E-1036.237085
GTTCGCA2206.2573235E-1032.939163
GTAGAAA7100.032.6698342
TAGTACT4550.031.8567714
AGTAGAA7300.031.7870831
GTATAGG1902.0726293E-730.532331
TTCGCAG2401.5879777E-930.1975654
CGCAGTT2901.2732926E-1129.9893076
TAGTACC2901.2732926E-1129.9893074
TGGTGCG1703.019668E-629.84237
TAGGGTG2003.2639218E-728.9896645
GTACTAA1555.4924654E-528.0700441
TATAGGG2405.2801624E-827.1823222
Produced by FastQC (version 0.11.5)