FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_E02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_E02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2441737
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCAT122600.5021015776883423No Hit
GTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGT75830.31055760714606034No Hit
GCTTTAGAGTGCCCTAATGCCTGTGAGCAAGCCCCTGGTCCCAGCTTCCA47270.19359169312665533No Hit
GTAAAAAAACCCCGCAAAAGTAAAAACAAAAACAAAAACAAAAACCCTTT37340.15292392260100085No Hit
CATTTAGGGAGGACCCACCCTGTGCCAGGCACTAGGTATGCAAATCTCCA36830.15083524556494005No Hit
GGGCTGAGGTGACCTCAGAGGCCAGCTGGTCCAAATCCCTCATTGCACAG36170.14813225175356723No Hit
GCAGAGACAGAGGACTGGAGAGCAAAAGGAGGTCCCTCAAGATCCCACAA33970.13912227238232455No Hit
CTCTAAAGCAGGAAAAGGAGGGAGGGAGGGAGGTTTACATTTAGGGAGGA33530.13732027650807602No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA33390.13674691418445148No Hit
GTATTACGACTGTCTCCATTTTACAGATGAATGGACTGAGACTGGAGGCG31810.13027611081783175No Hit
GTCGTAATACCTGTTCCTCAGGGTGGCTGAGAAGATTGAATGACATCAGG30430.12462439648496132No Hit
TTGTAATTGTGTGTTTGTGTTAGTCTCTGCTGCTAGATCCTGAGCGAGCT28980.11868600099027864No Hit
GTAGAAACAAGGACAGTGGGAAGTTGAGGGTTTCTGTGAGTTTGAAAATT28910.11839931982846638No Hit
GTTGAGGGTTTCTGTGAGTTTGAAAATTTGCATAATAAAAAGTAAAAAAA27740.11360764898103277No Hit
ATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCAGC27700.1134438311742829No Hit
CAATTACAAAGTGGTAAGTGGTTTATTTTCAAATATCAAAAACACTTTCA26080.10680921000091328No Hit
GACATGTACAAAGCACTTTATGTGCCTGATGTCATTCAATCTTCTCAGCC25330.10373762612435326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7000.070.434261
TACCGAT701.7010501E-551.763377
CGAAAGC9650.050.3212553
GACAGTG46600.048.9863667
GGACAGT46550.048.7266276
GCGAAAG10050.048.3273282
GTAGAAA20900.045.446251
ACGATAG657.228259E-444.7529145
CAAGGAC50700.040.1700443
ACAAGGA54200.037.3154752
AGGACAG65200.036.3447655
AACAAGG61100.035.3629231
ACAGTGG69650.034.33558
CAGTGGG63750.034.102939
TAGAAAC28700.033.0882262
AAGGACA66650.031.9680044
TTAGCGA5150.030.9613153
CGAAGTA5200.030.6592165
AGTAGAA18450.030.652881
GGTCGCG950.00465796430.620407145
Produced by FastQC (version 0.11.5)