FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_D10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_D10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979673
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG3850.039.537639
CGAAAGC2750.034.2729452
AGCGAAA1751.0081931E-733.1464541
GCGAAAG3200.031.722191
AGGTCGT3052.3646862E-1128.5189466
CCGTATG1357.9410715E-426.933167145
GACTATA1958.746876E-626.0284651
TAGAAAC9900.025.6288593
GTATAGT2559.4758434E-825.5910111
GTATAGG3201.2387318E-924.9245781
TTATACC6450.024.7250524
CCTTATA5900.023.3476072
TATAAGG4353.6379788E-1223.333482
TATAGAC4700.023.1360823
AGTAGAA9850.022.8197351
GGATAGC5150.022.5186655
TCGTATA1952.6145775E-422.3078332
TATACGT1952.617289E-422.3044194
TATACCA6900.022.0597255
TATACTG7300.021.8438765
Produced by FastQC (version 0.11.5)