FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_D09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_D09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324102
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA24510.1851065854443238No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA22190.16758527666297612No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC21080.1592022366856934No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT21080.1592022366856934No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG20480.15467086372500002No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA19340.14606125509968265No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG18520.13986837872006838No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT18010.13601671170347904No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC17360.13110772432939458No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT16590.1252924623631714No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC16460.12431066488835453No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA15880.11993033769301761No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT15230.11502135031893314No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG14810.11184938924644779No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT14530.10973474853145755No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT14520.10965922564877932No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG14380.1086019052912842No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG14210.10731801628575442No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA13720.10361739503452151No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT13660.10316425773845217No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG13550.10233350602899172No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13330.10067200261007082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG151.215818E-4145.42552145
GCGACCG203.821526E-4109.06914145
TAGTTCG203.821526E-4109.06914145
AGCGAAA1550.093.567611
TATGGCG259.2787744E-487.25532145
TGACGCG259.2787744E-487.25532145
AGATGCG353.388081E-583.1003145
TGGACCG809.640644E-1172.71276145
TATCGTG350.003570799362.1250957
CGAAAGC2350.061.6798363
CAATTCG4850.059.7767229
TCAACGG502.0133259E-457.9834188
TTATACT7950.055.6046334
CAACGGG553.223929E-452.7122049
TTCAACG553.223929E-452.7122047
ACAATTC6200.049.098878
TACATCG752.5072184E-548.475178145
ACACTCG450.00952886248.475174145
AGTAGAA6600.048.3432581
GGTACTG4350.048.315875
Produced by FastQC (version 0.11.5)