FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_D07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_D07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827340
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC16110.19472042932772501No Hit
GGCTTATCCTGAGGCTCCTAACCTGCCTTCAGCCAGGAGGGGAAATACGT11790.14250489520632387No Hit
GCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTCATTCCCTCCC11120.1344066526458288No Hit
GGTCAGGAGTGTCCTTCATTCCCTCCCTTGAGAGCAGATGTGTGAGGCCA10690.12920927309207825No Hit
TCTTGGAAGTGCTCTAGGAAGTGGCCCGAAGGAGGTCAGGAGTGTCCTTC9960.12038581477989703No Hit
GCATTATTGTATGCCCGCTGTGCCAGGCGCTGAGCCCAGTGTGCGTAGAT9950.12026494548794933No Hit
GACCTGGAGTGACCAGGACCAGCAGCTGGCGAGTCACTGCTGATGGCAAA9610.11615538956172794No Hit
GTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGGTGTGTGTTGT8640.10443106824280224No Hit
GTGTGGCACAGTGGTAGTGGCCGTGGGCTCTGACGGCACCCGGGTCTGGG8420.1017719438199531No Hit
GTTAATGGGATGTCATCTACGCACACTGGGCTCAGCGCCTGGCACAGCGG8410.10165107452800543No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2500.063.798831
GCGAAAG3000.055.5823142
TACCGAT450.00965307948.3149077
ATGAACG1203.6343408E-948.3119936
TAGCGCA450.00965769248.3090674
CGAAAGC3350.047.596673
GTAGAAA8600.041.3073841
ATATGCG850.002699924934.224678145
GCATTAA4200.032.7970161
AACGTCA2151.8153514E-830.3391049
TGAACGT1952.6151065E-729.732257
GTATATA3450.029.4197481
TAGAAAC12300.028.8815842
GAACGTC2054.0490704E-728.2836078
GGACAGT6350.027.3894756
GCTATTG4250.027.2853648
CCCTATA3005.857146E-1026.5828462
AGCGGGT1100.00968576526.355188
TAAACCG1100.00969719126.3488065
GTATGGC3409.094947E-1125.5784847
Produced by FastQC (version 0.11.5)