FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711065
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG224661.3129834342938462No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA164270.9600453518714953No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA151620.8861147881582524No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT149980.8765301142855474No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC131630.7692869645513175No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG115940.6775896882935482No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT107240.6267441622615155No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA107010.625399970194002No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG99850.5835546867009728No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT89740.5244686788637486No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC87100.5090396916540283No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC83870.4901625595754691No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG71860.4199723563979159No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC71230.41629043899559626No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT68740.40173809878642835No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC68560.4006861223857656No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA62880.367490422631519No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC62750.366730661897707No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG59640.34855484741958953No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA59590.3482626317527388No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG51170.29905351345507036No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT50510.2951962666526403No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC47890.27988416570966035No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG46850.2738060798391645No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG46710.2729878759719824No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC44280.2587861945630353No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT43690.25533804969419627No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA42920.25083792842469455No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT41670.2435325367534255No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA40530.23687001954922812No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG39600.23143480814580394No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC39230.22927241221110828No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG38940.22757756134337387No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG38880.22722690254315298No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATTCATCCTCAAGAATTC38790.22670091434282158No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA38770.2265840280760813No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA38280.22372031454094382No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC37750.22062282847232573No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC35790.20916797433177584No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT35110.20519384126260545No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA34510.2016872532603963No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG34510.2016872532603963No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT33840.1977715633245961No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTA33500.1957844967900109No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATATT33250.1943234184557571No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC32930.1924532381879122No Hit
GGACAAGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAA32460.18970641091951504No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG32450.18964796778614487No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA32450.18964796778614487No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG31010.1812321565808429No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA30890.1805308389804011No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG30830.18018018018018017No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC30760.1797710782465891No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG30760.1797710782465891No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT29860.17451119624327538No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG29480.1722903571752096No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTTTGTAATCCC29370.17164748270813793No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC27450.16042640110106862No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG27000.15779646009941176No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC26760.1563938248985281No Hit
GTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGA26280.1535885544967608No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA25930.15154304482880546No Hit
GTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAA24630.1439454374906856No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA24570.1435947786904647No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA24280.1418999278227303No Hit
ATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATA24090.14078950828869738No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG23890.13962064562129434No Hit
CCCCAGATGTGCCTTCATCTGGATCTTCAGTCAATGCACCTGCATCCTTT23880.1395622024879242No Hit
GATCTTATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA23040.1346529792848314No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA22890.1337763322842791No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT22040.12880866594781612No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG22040.12880866594781612No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACC21910.12804890521400414No Hit
AGTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGAT21880.1278735758138937No Hit
GTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTATCCTCAACCCTG21700.12682159941323096No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG21640.12647094061301No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA21630.12641249747963987No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCATCCTC21580.12612028181278911No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAA21490.12559429361245772No Hit
CGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAA20650.12068507040936492No Hit
GGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCG20500.11980842340881263No Hit
GCCATAAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGG20350.11893177640826035No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT20330.11881489014152005No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG20150.11776291374085729No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA20080.11735381180726623No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA19930.11647716480671394No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGAACTGTAAGTCGTTT19890.11624339227323333No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG19830.11589273347301242No Hit
GAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCAAAAT19820.11583429033964227No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT19210.11226925920406296No Hit
GGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGA19180.11209392980395252No Hit
GTGTCAAATGTCCCACTTCAATATAAACACTGCCTGTTCCGCCGGCTACT19160.1119770435372122No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC18850.11016530640273747No Hit
GAATGAAGTGGATGATGGCAATGAGATAGAGTAGGATGCAATTTTCCTCA18560.10847045553500306No Hit
CAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG18430.10771069480119107No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATT18360.1073015928676No Hit
GGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATGGCAATGAGA18200.10636650273367758No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG18150.10607428706682681No Hit
ATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCGCA18030.10537296946638497No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT17920.1047300949993133No Hit
GCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACT17430.10186638146417583No Hit
GACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATGGCCTC17400.10169105206406537No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT17310.10116506386373399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA34600.0105.038961
AGCGAAA4500.0104.782721
CGACGAG301.5730797E-596.99492145
GTCGACG651.4551915E-1189.53378145
GCAGGTA8900.088.812252
CGTATCG259.262312E-487.29543145
CATTGCG502.2168024E-887.29543145
GATTACG259.262312E-487.29543145
CAGGTAC9500.083.178733
GAGCGAA801.8189894E-1281.609611
GGTCGCG451.2086475E-680.8291145
GGTCAAA17600.080.37311
CGACTCG554.2940883E-879.35948145
TTGCTCG651.5243131E-978.34206145
ACTGATC10450.077.675528
GCGAAAG6200.076.025282
CGAAAGC6200.076.003053
CGGACCG300.001910115972.74619145
GTACTGA11950.072.168686
GTAGAAA51100.070.955412
Produced by FastQC (version 0.11.5)