FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744193
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG901.9983418E-856.573925145
CGTATCG752.50243E-548.491936145
AGCGAAA5100.044.067371
TTATACG1303.6066194E-739.166565145
CGAAAGC6050.038.333923
CAATTCG6350.037.6609659
CAGCTCG8400.036.2344179
TATACAG11600.031.2338775
TATATAC22600.027.8996933
TAAGGCG1306.5044954E-427.870235
GTATACG3403.6379788E-1227.7197991
CCTTATA9450.026.8472352
TATACTT14450.024.572095
GCGAAAG9900.023.4303132
CATATAT16950.023.0966871
GTAGAAA19650.022.8746661
GTATAGG3503.5070116E-922.7850671
TACTTAC14000.022.7746127
CTTACAC12550.022.5201939
GGATAGC6150.022.386825
Produced by FastQC (version 0.11.5)