FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C10_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C10_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250295
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG30180.24138303360406946No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT27410.2192282621301373No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA22060.17643836054691092No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA15380.12301096941121895No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT13620.1089342915072043No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA13250.10597498990238305No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG151.2143918E-4145.4679145
AGCGAAA2750.0110.702281
TGACGCG651.4551915E-1189.5187145
CGACTCG353.383114E-583.12452145
GGTCGCG451.2096643E-680.8155145
CGAAAGC4500.067.635153
GACGACG553.985253E-666.12177145
AGCGAAT502.013879E-457.9799426
TCGAGCG400.005974599654.55046145
AGTAGCG400.005974599654.55046145
GAGCGAA805.884067E-754.362721
TTATACG901.2947694E-648.4893145
TTGAGCG604.8676023E-448.4893145
TATTAGG2300.044.1169432
CCGTAGT855.349383E-542.6374321
TGTCTCG1401.3753379E-841.56226145
TAGCGAA700.001059375441.4076235
GCGAAAG7550.040.3187982
CAGCTCG12550.039.8467759
GTATTAC4050.039.3738251