FastQCFastQC Report
Sat 5 Dec 2020
H7KKVBGXH_n02_S5_R2_C08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7KKVBGXH_n02_S5_R2_C08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737288
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC10810.1466184177689044No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT9480.12857933399160165No Hit
CTATTCCACCGTGCTAACAGTCCCTTTGAGAACTCAGCCATCCATTACAC8700.11800002170115342No Hit
GTGTACACCCAGCCTTTGAACATTTTTATCTGTGGCTGCATTCATGCCAG7940.10769197382840899No Hit
CACACAGGAGCTATCAATCACTCTCGTCTCCCTGGTTTCTGTATGCACTC7840.10633565173988997No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG300.001909357472.749306145
CGAAAGC2100.048.3217963
AGCGAAA2050.045.9896051
GCATTAA4350.045.01371
GCGAAAG2450.041.4299282
GTATATA3100.032.7519041
AAATGCG1153.0982788E-431.63013145
CATTAAG6300.031.0724452
GATTAGC2601.10958354E-1030.6824131
TGAAGCG1000.00597240829.099722145
GTCTAGT2802.6193447E-1028.4908141
GTAGAAA6700.024.8888762
AGTAGAA6550.024.3585571
ACCGTGC6050.023.954728
TATATAG3753.074092E-1023.200762
GTATAGA2852.7685928E-722.9017071
TATAGTA3554.118192E-922.4655272
GGGTAAG2603.1938143E-622.3144821
TAGCACA3955.9117156E-1022.0170734
TAAGATG9650.021.7764535
Produced by FastQC (version 0.11.5)